Caspase 2/p18 Polyklonaler Antikörper

Caspase 2/p18 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24777-1-AP

Synonyme

CASP 2, CASP2, Caspase 2, Caspase 2/p18, Caspase2, ICH 1L, ICH 1L/1S, ICH1, NEDD 2, NEDD2, Protease ICH 1

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 24777-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24777-1-AP (Caspase 2/p18 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 24777-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 24777-1-AP (Caspase 2/p18 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 24777-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 24777-1-AP (Caspase 2/p18 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 24777-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24777-1-AP (Caspase 2/p18 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 24777-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24777-1-AP (Caspase 2/p18 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 24777-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 24777-1-AP (Caspase 2/p18 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 24777-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 24777-1-AP (Caspase 2/p18 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 24777-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 24777-1-AP (Caspase 2/p18 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Jurkat-Zellen, Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

24777-1-AP bindet in WB, IHC, IF, ELISA Caspase 2/p18 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Caspase 2/p18 fusion protein Ag20141
• Vollständiger Name: caspase 2, apoptosis-related cysteine peptidase
• Berechnetes Molekulargewicht: 452 aa, 51 kDa
• Beobachtetes Molekulargewicht: 48-51 kDa, 32 kDa
• GenBank-Zugangsnummer: BC002427
• Gene symbol: CASP2
• Gene ID (NCBI): 835
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CASP2(Caspase-2) is also named as ICH1, NEDD2 and belongs to the peptidase C14A family.It is involved in the activation cascade of caspases responsible for apoptosis execution and might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival.It has 2 isoforms produced by alternative splicing and can exists almost as a dimer in solution(PMID:15865942).