cleaved-Caspase 3 Polyklonaler Antikörper

cleaved-Caspase 3 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

128

Kat-Nr. : 25546-1-AP

Synonyme

cleaved-Caspase 3

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 25546-1-AP

  Tunicamycin treated HepG2 cells were subjected to SDS PAGE followed by western blot with 25546-1-AP (Caspase 3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 25546-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25546-1-AP (Caspase 3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 25546-1-AP

  Tunicamycin treated HeLa cells were subjected to SDS PAGE followed by western blot with 25546-1-AP (Caspase 3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 25546-1-AP

  Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 25546-1-AP (cleaved-caspase 3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 25546-1-AP

  IP Result of anti-Caspase 3 (IP:25546-1-AP, 4ug; Detection:25546-1-AP 1:300) with Jurkat cells lysate 3200ug.

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  IHC staining of human skeletal muscle using 25546-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 25546-1-AP (Caspase 3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 25546-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 25546-1-AP (Caspase 3 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 25546-1-AP

  Immunofluorescent analysis of HepG2 cells using 25546-1-AP (Caspase 3 Antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 25546-1-AP

  1X10^6 HeLa cells were stained with 0.2ug Caspase 3 antibody (25546-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: mit Tunicamycin behandelte HepG2-Zellen, mit Staurosporin behandelte Jurkat-Zellen, mit Tunicamycin behandelte HeLa-Zellen
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 108 publications below
• IHC: See 9 publications below
• IF: See 4 publications below
• FC: See 1 publications below

Produktinformation

25546-1-AP bindet in WB, IP, IHC, IF, FC, ELISA cleaved-Caspase 3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, hamster, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: caspase 3, apoptosis-related cysteine peptidase
• Berechnetes Molekulargewicht: 32 kDa
• Beobachtetes Molekulargewicht: 17 kDa
• GenBank-Zugangsnummer: NM_032991
• Gene symbol: CASP3
• Gene ID (NCBI): 836
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Caspase 3, also named CPP32, SCA-1, and Apopain, belongs to the peptidase C14A family. Caspase 3 is involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis, it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Caspase 3 cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7, and -9. CASP3 is involved in the cleavage of huntingtin. The antibody can recognize active-caspase 3.

Publikationen

Species	Application	Title
human		Nat Methods; DART-seq: an antibody-free method for global m6A detection.; Authors - Kate D Meyer
human	WB	Autophagy; Increased mitochondrial fission promotes autophagy and hepatocellular carcinoma cell survival through the ROS-modulated coordinated regulation of the NFKB and TP53 pathways.; Authors - Qichao Huang
mouse	WB	Diabetes; MICU1 Alleviates Diabetic Cardiomyopathy Through Mitochondrial Ca(2+)-dependent Antioxidant Response.; Authors - Lele Ji
		PLoS Biol; The Machado-Joseph Disease Deubiquitinase Ataxin-3 Regulates the Stability and Apoptotic Function of p53.; Authors - Hongmei Liu
human	WB	Biol Res; MicroRNA-1247 inhibits cell proliferation by directly targeting ZNF346 in childhood neuroblastoma.; Authors - Tingting Wu
rat	WB	Oxid Med Cell Longev; Classic Prescription, Kai-Xin-San, Ameliorates Alzheimer's Disease as an Effective Multitarget Treatment: From Neurotransmitter to Protein Signaling Pathway.; Authors - Sirui Guo