Caspase 3/P17/P19 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

Caspase 3/P17/P19 Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (3)

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(43)

CloneNo.

5G20

Kat-Nr. : 82202-1-RR

Synonyme

CASP3, Caspase3, 5G20, CASP 3, CASP-3

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  WB analysis of Jurkat using 82202-1-RR

  Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 82202-1-RR (Caspase 3/P17/19 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 82202-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82202-1-RR (Caspase 3/P17/19 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 82202-1-RR

  WB result of Caspase 3/P17/P19 antibody (82202-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Caspase 3/P17/P19 transfected HeLa cells.

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  IHC staining of mouse brain using 82202-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82202-1-RR (Caspase 3/P17/19 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 82202-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82202-1-RR (Caspase 3/P17/19 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 82202-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82202-1-RR (Caspase 3/P17/19 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 82202-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82202-1-RR (Caspase 3/P17/19 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 82202-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82202-1-RR (Caspase 3/P17/19 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 82202-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82202-1-RR (Caspase 3/P17/19 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 82202-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82202-1-RR (Caspase 3/P17/19 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 82202-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Caspase 3/P17/19 antibody (82202-1-RR, Clone: 5G20 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of HeLa using 82202-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Caspase 3/P17/19 antibody (82202-1-RR, Clone: 5G20 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HepG2 using 82202-1-RR

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Caspase 3/P17/19 (82202-1-RR, Clone:5G20) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: mit Staurosporin behandelte Jurkat-Zellen, HeLa-Zellen, HepG2-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:500-1:2000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 36 publications below
• IHC: See 7 publications below
• IF: See 4 publications below

Produktinformation

82202-1-RR bindet in WB, IHC, IF/ICC, FC (Intra), ELISA Caspase 3/P17/P19 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Kaninchen, Maus, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: caspase 3, apoptosis-related cysteine peptidase
• Berechnetes Molekulargewicht: 32 kDa
• Beobachtetes Molekulargewicht: 32-35 kDa, 17 kDa, 19 kDa
• GenBank-Zugangsnummer: NM_004346
• Gene symbol: Caspase 3
• Gene ID (NCBI): 836
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 (also named CPP32, SCA-1, and Apopain) proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at the beginning of apoptosis. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can also form heterocomplex with other proteins and performs the molecular mass of 50-70 kDa. This antibody can recognize p17, p19 and p32 of Caspase 3.

Publikationen

Species	Application	Title
mouse	WB	Int Immunopharmacol; CSF1R inhibition agents protect against cisplatin ototoxicity and synergize with immunotherapy for Head and Neck Squamous Cell Carcinoma; Authors - Jiahui Zhang
human	WB	Acta Biochim Biophys Sin (Shanghai); Magnolol promotes the autophagy of esophageal carcinoma cells by upregulating HACE1 gene expression; Authors - Kenan Huang
bovine	IF	Biomol Biomed; A repeated strike loading organ culture model for studying compression-associated chronic disc degeneration; Authors - Baoliang Li
human	WB	Int J Chron Obstruct Pulmon Dis; Effective-Component Compatibility of Bufei Yishen Formula III Suppresses Mitochondrial Oxidative Damage in COPD: Via Pkm2/Nrf2 Pathway; Authors - Yang Liu
mouse	IHC	Biomater Res; Genetically Modified Hepatocytes Targeting Bilirubin and Ammonia Metabolism for the Construction of Bioartificial Liver System; Authors - Ke Wang
human	WB	Front Chem; New molecular mechanisms of quercetin in improving recurrent spontaneous abortion based on in-depth network pharmacology and molecular docking; Authors - Dan Wang