Caspase 3/P17/P19 Monoklonaler Antikörper

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• KD/KO Validated

Caspase 3/P17/P19 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2G4B2

Kat-Nr. : 66470-2-PBS

Synonyme

CASP3, Caspase3, 2G4B2, CASP 3, CASP-3

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  WB analysis of HEK-293 using 66470-2-Ig (same clone as 66470-2-PBS)

  WB result of Caspase 3 antibody (66470-2-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Caspase 3 transfected HEK-293 cells. This data was developed using the same antibody clone with 66470-2-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 66470-2-Ig (same clone as 66470-2-PBS)

  Untreated and Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 66470-2-Ig (CASP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66470-2-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66470-2-Ig (same clone as 66470-2-PBS)

  WB result of Caspase 3/P17/P19 antibody (66470-2-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Caspase 3/P17/P19 transfected HeLa cells. This data was developed using the same antibody clone with 66470-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 66470-2-Ig (same clone as 66470-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66470-2-Ig (Caspase 3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66470-2-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 66470-2-Ig (same clone as 66470-2-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66470-2-Ig (CASP3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66470-2-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66470-2-Ig (same clone as 66470-2-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66470-2-Ig (CASP3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66470-2-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 66470-2-Ig (same clone as 66470-2-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66470-2-Ig (CASP3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66470-2-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 66470-2-Ig (same clone as 66470-2-PBS)

  Untreated and Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 66470-2-Ig (CASP3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66470-2-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66470-2-Ig (same clone as 66470-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66470-2-Ig (CASP3 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66470-2-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66470-2-Ig (same clone as 66470-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66470-2-Ig (CASP3 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66470-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 66470-2-Ig (same clone as 66470-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66470-2-Ig (Caspase 3 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66470-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 66470-2-Ig (same clone as 66470-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66470-2-Ig (Caspase 3 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66470-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 66470-2-Ig (same clone as 66470-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66470-2-Ig (Caspase 3 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66470-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 66470-2-Ig (same clone as 66470-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66470-2-Ig (Caspase 3 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66470-2-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66470-2-Ig (same clone as 66470-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Caspase 3/p17/p19 antibody (66470-2-Ig, Clone: 2G4B2 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66470-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66470-2-PBS bindet in WB, IHC, IF/ICC, ELISA Caspase 3/P17/P19 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Caspase 3/P17/P19 fusion protein Ag25029
• Vollständiger Name: caspase 3, apoptosis-related cysteine peptidase
• Berechnetes Molekulargewicht: 277 aa, 32 kDa
• Beobachtetes Molekulargewicht: 32-35 kDa, 19 kDa, 17 kDa
• GenBank-Zugangsnummer: BC016926
• Gene symbol: Caspase 3
• Gene ID (NCBI): 836
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 (also named CPP32, SCA-1, and Apopain) proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at the beginning of apoptosis. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can also form heterocomplex with other proteins and performs the molecular mass of 50-70 kDa. This antibody can recognize p17, p19 and p32 of Caspase 3.