Caspase 4 Monoklonaler Antikörper

Caspase 4 Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1D1E12

Kat-Nr. : 67398-1-PBS

Synonyme

CASP4, Caspase4, 1D1E12, CASP 4, CASP-4

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  WB analysis of SGC-7901 using 67398-1-Ig (same clone as 67398-1-PBS)

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 67398-1-Ig (Caspase 4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67398-1-PBS in a different storage buffer formulation.

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  WB analysis of HL-60 using 67398-1-Ig (same clone as 67398-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 67398-1-Ig (Caspase 4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67398-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 67398-1-Ig (same clone as 67398-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 67398-1-Ig (Caspase 4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67398-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 67398-1-Ig (same clone as 67398-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67398-1-Ig (Caspase 4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67398-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67398-1-Ig (same clone as 67398-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67398-1-Ig (Caspase 4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67398-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67398-1-Ig (same clone as 67398-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67398-1-Ig (Caspase 4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67398-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67398-1-Ig (same clone as 67398-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67398-1-Ig (Caspase 4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67398-1-PBS in a different storage buffer formulation.

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  IF Staining of human liver cancer using 67398-1-Ig (same clone as 67398-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Caspase 4 antibody (67398-1-Ig, Clone: 1D1E12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67398-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human liver cancer using 67398-1-Ig (same clone as 67398-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Caspase 4 antibody (67398-1-Ig, Clone: 1D1E12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67398-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 67398-1-Ig (same clone as 67398-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Caspase 4 antibody (67398-1-Ig, Clone: 1D1E12 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67398-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67398-1-PBS bindet in WB, IHC, IF/ICC, IF-P, Indirect ELISA Caspase 4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Caspase 4 fusion protein Ag29488
• Vollständiger Name: caspase 4, apoptosis-related cysteine peptidase
• Berechnetes Molekulargewicht: 377 aa, 43 kDa
• Beobachtetes Molekulargewicht: 43-48 kDa
• GenBank-Zugangsnummer: BC017839
• Gene symbol: Caspase 4
• Gene ID (NCBI): 837
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Caspase 4 (CASP4), is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis.Caspase 4 is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. Overexpression of caspase 4 will induce cell apoptosis.