Caspase 6 Monoklonaler Antikörper

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• KD/KO Validated

Caspase 6 Monoklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

1H7F9

Kat-Nr. : 67825-1-Ig

Synonyme

Apoptotic protease Mch 2, CASP 6, CASP6, Caspase 6, Caspase6, MCH2

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Galerie der Validierungsdaten

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  WB analysis using 67825-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67825-1-Ig (Caspase 6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 67825-1-Ig

  WB result of Caspase 6 antibody (67825-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Caspase 6 transfected Jurkat cells.

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  IF Staining of Jurkat using 67825-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using Caspase 6 antibody (67825-1-Ig, Clone: 1H7F9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen
• Erfolgreiche Detektion in IF: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunfluoreszenz (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67825-1-Ig bindet in WB, IF, ELISA Caspase 6 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Caspase 6 fusion protein Ag20534
• Vollständiger Name: caspase 6, apoptosis-related cysteine peptidase
• Berechnetes Molekulargewicht: 33 kDa, 22 kDa
• Beobachtetes Molekulargewicht: 33-35 kDa，22kDa, 18kDa
• GenBank-Zugangsnummer: BC000305
• Gene symbol: CASP6
• Gene ID (NCBI): 839
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Caspase-6 belongs to caspase family of cysteinyl-aspartate specific proteases.Precursor of CASP6 produces two subunits, large (18kDa) and small (16kDa) that dimerize. It cleaves poly(ADP-ribose) polymerase, as well as lamins and is involved in the activation cascade of caspases responsible for apoptosis execution. Researches showed that CASP6 could be an early instigator of neuronal dysfunction and regulates B cell activation and differentiation into plasma cells by modifying cell cycle entry. IRAK3 is an important target for CASP6. It can reveal five bands of 28, 32, 36, 49, and 64 kDa in human neurons and fetal brain in western blot, the 32 and 28 kDa bands represent procaspase-6 and pro-arm caspase-6. Procaspase-6 is more abundant than pro-arm caspase-6 in adult tissue, whereas pro-arm caspase-6 is more abundant than pro-caspase-6 in fetal brain and cultured neurons. The higher molecular mass bands at 49 and 64 kDa likely represent dimers of p28 and p32.(PMID:10438520). In rat testis, it can be detected two bands of 34 kDa and 12 kDa or 14 kDa(PMID:12538628).