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Caspase 6 Monoklonaler Antikörper

Caspase 6 Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1H7F9

Kat-Nr. : 67825-1-PBS

Synonyme

Apoptotic protease Mch 2, CASP 6, CASP6, Caspase 6, Caspase6, MCH2

Filter:
  • Western Blot (WB) analysis of various lysates using Caspase 6 Monoclonal antibody (67825-1-Ig)

    WB analysis using 67825-1-Ig (same clone as 67825-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67825-1-Ig (Caspase 6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67825-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using Caspase 6 Monoclonal antibody (67825-1-Ig)

    WB analysis of Jurkat using 67825-1-Ig (same clone as 67825-1-PBS)

    WB result of Caspase 6 antibody (67825-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Caspase 6 transfected Jurkat cells. This data was developed using the same antibody clone with 67825-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using Caspase 6 Monoclonal antibody (67825-1-Ig)

    IF Staining of Jurkat using 67825-1-Ig (same clone as 67825-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using Caspase 6 antibody (67825-1-Ig, Clone: 1H7F9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67825-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

67825-1-PBS bindet in WB, IF/ICC, Indirect ELISA Caspase 6 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG2b
Klonalität Monoklonal
Typ Antikörper
Immunogen Caspase 6 fusion protein Ag20534
Vollständiger Name caspase 6, apoptosis-related cysteine peptidase
Berechnetes Molekulargewicht 33 kDa, 22 kDa
Beobachtetes Molekulargewicht 33-35 kDa，22kDa, 18kDa
GenBank-ZugangsnummerBC000305
Gene symbol Caspase 6
Gene ID (NCBI) 839
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Caspase-6 belongs to caspase family of cysteinyl-aspartate specific proteases.Precursor of CASP6 produces two subunits, large (18kDa) and small (16kDa) that dimerize. It cleaves poly(ADP-ribose) polymerase, as well as lamins and is involved in the activation cascade of caspases responsible for apoptosis execution. Researches showed that CASP6 could be an early instigator of neuronal dysfunction and regulates B cell activation and differentiation into plasma cells by modifying cell cycle entry. IRAK3 is an important target for CASP6. It can reveal five bands of 28, 32, 36, 49, and 64 kDa in human neurons and fetal brain in western blot, the 32 and 28 kDa bands represent procaspase-6 and pro-arm caspase-6. Procaspase-6 is more abundant than pro-arm caspase-6 in adult tissue, whereas pro-arm caspase-6 is more abundant than pro-caspase-6 in fetal brain and cultured neurons. The higher molecular mass bands at 49 and 64 kDa likely represent dimers of p28 and p32.(PMID:10438520). In rat testis, it can be detected two bands of 34 kDa and 12 kDa or 14 kDa(PMID:12538628).