Caspase 6 Rekombinanter Antikörper

Caspase 6 Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242457A8

Kat-Nr. : 85201-2-PBS

Synonyme

CASP6, Caspase6, 242457A8, Apoptotic protease Mch 2, Apoptotic protease Mch-2

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  WB analysis of Jurkat using 85201-2-RR (same clone as 85201-2-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 85201-2-RR (Caspase 6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85201-2-PBS in a different storage buffer formulation.

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  IHC staining of Jurkat using 85201-2-RR (same clone as 85201-2-PBS)

  Immunohistochemical analysis of paraffin-embedded Jurkat cells slide using 85201-2-RR (Caspase 6 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with . This data was developed using the same antibody clone with 85201-2-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 85201-2-RR (same clone as 85201-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Staurosporine treated Jurkat cells using Caspase 6 antibody (85201-2-RR, Clone: 242457A8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85201-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

85201-2-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA Caspase 6 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: caspase 6, apoptosis-related cysteine peptidase
• Berechnetes Molekulargewicht: 33 kDa, 22 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: BC000305
• Gene symbol: Caspase 6
• Gene ID (NCBI): 839
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Caspase-6 belongs to caspase family of cysteinyl-aspartate specific proteases.Precursor of CASP6 produces two subunits, large (18kDa) and small (16kDa) that dimerize. It cleaves poly(ADP-ribose) polymerase, as well as lamins and is involved in the activation cascade of caspases responsible for apoptosis execution. Researches showed that CASP6 could be an early instigator of neuronal dysfunction and regulates B cell activation and differentiation into plasma cells by modifying cell cycle entry. IRAK3 is an important target for CASP6. It can reveal five bands of 28, 32, 36, 49, and 64 kDa in human neurons and fetal brain in western blot, the 32 and 28 kDa bands represent procaspase-6 and pro-arm caspase-6. Procaspase-6 is more abundant than pro-arm caspase-6 in adult tissue, whereas pro-arm caspase-6 is more abundant than pro-caspase-6 in fetal brain and cultured neurons.