Caspase 7 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Caspase 7 Polyklonal Antikörper für WB, IF/ICC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (3)

Anwendung

WB, IHC, IF/ICC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(71)

Kat-Nr. : 27155-1-AP

Synonyme

CASP7, Apoptotic protease Mch 3, Apoptotic protease Mch-3, CASP 7, CASP-7

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  WB analysis using 27155-1-AP

  Untreated and Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 27155-1-AP (Caspase 7 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 27155-1-AP

  WB result of Caspase 7 antibody (27155-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-Caspase 7 transfected HEK-293 cells.

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  WB analysis of HEK-293 using 27155-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 27155-1-AP (Caspase 7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of LNCaP using 27155-1-AP

  LNCaP cells were subjected to SDS PAGE followed by western blot with 27155-1-AP (Caspase 7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of LNCaP using 27155-1-AP

  LNCaP cells were subjected to SDS PAGE followed by western blot with 27155-1-AP (Caspase 7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 27155-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 27155-1-AP (Caspase 7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 27155-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 27155-1-AP (Caspase 7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 27155-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 27155-1-AP (Caspase 7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 27155-1-AP

  IP result of anti-Caspase 7 (IP:27155-1-AP, 4ug; Detection:27155-1-AP 1:1000) with HEK-293 cells lysate 2600 ug.

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  IF Staining of mouse brain using 27155-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Caspase 7 antibody (27155-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brain using 27155-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Caspase 7 antibody (27155-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 27155-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Caspase 7 antibody (27155-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of MCF-7 using 27155-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Caspase 7 antibody (27155-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HEK-293-Zellen, LNCaP-Zellen, MCF-7-Zellen, Mauslebergewebe, PC-3-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IF-P: Maushirngewebe
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 67 publications below
• IHC: See 4 publications below
• IP: See 1 publications below

Produktinformation

27155-1-AP bindet in WB, IHC, IF/ICC, IF-P, IP, ELISA Caspase 7 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Caspase 7 fusion protein Ag25904
• Vollständiger Name: caspase 7, apoptosis-related cysteine peptidase
• Berechnetes Molekulargewicht: 303 aa, 34 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC015799
• Gene symbol: Caspase 7
• Gene ID (NCBI): 840
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Caspase 7(CASP7), like caspases 3 and 6, contains a short prodomain and, upon apoptotic induction, the 35 kDa proform is converted into a 32 kDa intermediate or preactive form which is further processed into two active subunits consisting of the p20 or large (18 kDa) subunit and the p10 or small (11 kDa) subunit and it is present in the brain, which is up-regulated and activated after traumatic injury(PMID:15953353). Caspase-7 is classified as a member of the subgroup of cysteine proteases most related to the Caenorhabditis elegans factor CED-3, which also includes caspase-3, -6, and -9(PMID:9426061). The protein is involved in the activation cascade of caspases responsible for apoptosis execution.

Publikationen

Species	Application	Title
rat	WB	Nat Commun; Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model; Authors - Mengyan Hu
human	WB	J Pineal Res; Melatonin synergizes the chemotherapeutic effect of 5-fluorouracil in colon cancer by suppressing PI3K/AKT and NF-κB/iNOS signaling pathways.; Authors - Yue Gao
human	WB	Cell Rep; Inhibiting G6PD by quercetin promotes degradation of EGFR T790M mutation; Authors - Zehe Ge
human	WB	Front Immunol; Identification of molecular subtypes based on PANoptosis-related genes and construction of a signature for predicting the prognosis and response to immunotherapy response in hepatocellular carcinoma; Authors - Jinfeng Zhu
human	WB	Phytother Res; Targeting HSP90 with picropodophyllin suppresses gastric cancer tumorigenesis by disrupting the association of HSP90 and AKT; Authors - Xiaoli Li
human	WB	Cell Death Discov; IDO1 inhibits ferroptosis by regulating FTO-mediated m6A methylation and SLC7A11 mRNA stability during glioblastoma progression; Authors - Qianting Tian