Caspase 7 Monoklonaler Antikörper

Caspase 7 Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

3C9H4

Kat-Nr. : 67956-1-Ig

Synonyme

Apoptotic protease Mch 3, CASP 7, CASP7, Caspase 7, Caspase7, CMH 1, ICE LAP3, ICE like apoptotic protease 3, MCH3

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Galerie der Validierungsdaten

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  WB analysis using 67956-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67956-1-Ig (Caspase 7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 67956-1-Ig

  PC-12 cells were subjected to SDS PAGE followed by western blot with 67956-1-Ig (Caspase 7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 67956-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 67956-1-Ig (Caspase 7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 67956-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67956-1-Ig (Caspase 7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67956-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67956-1-Ig (Caspase 7 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 67956-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67956-1-Ig (Caspase 7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse stomach using 67956-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 67956-1-Ig (Caspase 7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 67956-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 67956-1-Ig (Caspase 7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat stomach using 67956-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 67956-1-Ig (Caspase 7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat stomach using 67956-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 67956-1-Ig (Caspase 7 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, PC-12-Zellen, RAW 264.7-Zellen
• Erfolgreiche Detektion in IHC: Ratten-Magengewebe, humanes Leberkarzinomgewebe, Mauslebergewebe, Maus-Magengewebe, Rattenlebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67956-1-Ig bindet in WB, IHC, ELISA Caspase 7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Caspase 7 fusion protein Ag27601
• Vollständiger Name: caspase 7, apoptosis-related cysteine peptidase
• Berechnetes Molekulargewicht: 303 aa, 34 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC015799
• Gene symbol: CASP7
• Gene ID (NCBI): 840
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Caspase 7(CASP7), like caspases 3 and 6, contains a short prodomain and, upon apoptotic induction, the 35 kDa proform is converted into a 32 kDa intermediate or preactive form which is further processed into two active subunits consisting of the p20 or large (18 kDa) subunit and the p10 or small (11 kDa) subunit and it is present in the brain, which is up-regulated and activated after traumatic injury(PMID:15953353). Caspase-7 is classified as a member of the subgroup of cysteine proteases most related to the Caenorhabditis elegans factor CED-3, which also includes caspase-3, -6, and -9(PMID:9426061). The protein is involved in the activation cascade of caspases responsible for apoptosis execution.