Cleaved Caspase 8/P18 Rekombinanter Antikörper

Cleaved Caspase 8/P18 Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242445E5

Kat-Nr. : 85171-3-PBS

Synonyme

CASP8, Caspase 8, 242445E5, Apoptotic cysteine protease, Apoptotic protease Mch-5

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  WB analysis using 85171-3-RR (same clone as 85171-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85171-3-RR (Cleaved Caspase 8/P18 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85171-3-PBS in a different storage buffer formulation.

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  IHC staining of Jurkat using 85171-3-RR (same clone as 85171-3-PBS)

  Immunohistochemical analysis of paraffin-embedded Jurkat cells slide using 85171-3-RR (Cleaved Caspase 8/P18 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85171-3-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

85171-3-PBS bindet in WB, IHC, Indirect ELISA Cleaved Caspase 8/P18 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: caspase 8, apoptosis-related cysteine peptidase
• Berechnetes Molekulargewicht: 538 aa, 62 kDa
• Beobachtetes Molekulargewicht: 55 kDa, 18 kDa
• GenBank-Zugangsnummer: BC028223
• Gene symbol: Caspase 8
• Gene ID (NCBI): 841
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CASP8, also named as MCH5, MACH, FLICE, and CAP4, belongs to the peptidase C14A family. It may participate in the GZMB apoptotic pathways and functions as an upstream regulator in a-bisabolol-induced apoptosis. CASP8 catalyzes an essential intermediate step in the ubiquitination and proteasome-mediated degradation of IRF3(PMID:21816816 ). It may control diabetic embryopathy-associated apoptosis via regulation of the Bid-stimulated mitochondrion/caspase-9 pathway (PMID:19194987). This antibody recognize the cleaved caspase 8.