Caspase 8/P43/P18 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Caspase 8/P43/P18 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13423-1-PBS

Synonyme

CASP8, Caspase 8, Apoptotic cysteine protease, Apoptotic protease Mch-5, CAP4

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  WB analysis of Jurkat using 13423-1-AP (same clone as 13423-1-PBS)

  Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 13423-1-AP (Caspase 8/P43/P18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13423-1-PBS in a different storage buffer formulation.

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  WB analysis of UV treated Hela using 13423-1-AP (same clone as 13423-1-PBS)

  UV treated Hela cells were subjected to SDS PAGE followed by western blot with 13423-1-AP (Caspase 8/P43/P18 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13423-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW264.7 using 13423-1-AP (same clone as 13423-1-PBS)

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 13423-1-AP (Caspase 8/P43/P18 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13423-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 13423-1-AP (same clone as 13423-1-PBS)

  IP result of anti-Caspase 8/P43/P18 (IP:13423-1-AP, 3ug; Detection:13423-1-AP 1:500) with HeLa cells lysate 2500ug. This data was developed using the same antibody clone with 13423-1-PBS in a different storage buffer formulation.

• 

  IP experiment of Jurkat using 13423-1-AP (same clone as 13423-1-PBS)

  IP result of anti-Caspase 8/P43/P18 (IP:13423-1-AP, 4ug; Detection:13423-1-AP 1:500) with Jurkat cells lysate 2880 ug. This data was developed using the same antibody clone with 13423-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 13423-1-AP (same clone as 13423-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13423-1-AP (Caspase 8/P43/P18 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13423-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 13423-1-AP (same clone as 13423-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13423-1-AP (Caspase 8/P43/P18 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13423-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 13423-1-AP (same clone as 13423-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13423-1-AP (Caspase 8/P43/P18 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13423-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 13423-1-AP (same clone as 13423-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon using 13423-1-AP (Caspase 8/P43/P18 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 13423-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 13423-1-AP (same clone as 13423-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon using 13423-1-AP (Caspase 8/P43/P18 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13423-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 13423-1-AP (same clone as 13423-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Caspase 8/P43/P18 antibody (13423-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 13423-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13423-1-PBS bindet in WB, IHC, IF/ICC, IP, ELISA Caspase 8/P43/P18 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Caspase 8/P43/P18 fusion protein Ag4214
• Vollständiger Name: caspase 8, apoptosis-related cysteine peptidase
• Berechnetes Molekulargewicht: 538 aa, 62 kDa
• Beobachtetes Molekulargewicht: 53-57 kDa, 32-45 kDa
• GenBank-Zugangsnummer: BC028223
• Gene symbol: Caspase 8
• Gene ID (NCBI): 841
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CASP8, also named as MCH5, MACH, FLICE, and CAP4, belongs to the peptidase C14A family. It may participate in the GZMB apoptotic pathways and functions as an upstream regulator in a-bisabolol-induced apoptosis. CASP8 catalyzes an essential intermediate step in the ubiquitination and proteasome-mediated degradation of IRF3(PMID:21816816 ). It may control diabetic embryopathy-associated apoptosis via regulation of the Bid-stimulated mitochondrion/caspase-9 pathway (PMID:19194987). CASP8 is expressed as nine isoforms by alternative splicing with the molecular mass from 26 kDa to 62 kDa. This antibody can recognize the pro- and cleaved-caspase 8.