Caspr2/CNTNAP2 Rekombinanter Antikörper

Caspr2/CNTNAP2 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242391A9

Kat-Nr. : 85116-4-RR

Synonyme

CNTNAP2, 242391A9, CASPR2, Contactin-associated protein-like 2

---

• 

  WB analysis using 85116-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85116-4-RR (CNTNAP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat brain using 85116-4-RR

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 85116-4-RR (Caspr2/CNTNAP2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 85116-4-RR

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 85116-4-RR (Caspr2/CNTNAP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 85116-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85116-4-RR (Caspr2/CNTNAP2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 85116-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85116-4-RR (Caspr2/CNTNAP2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Affinity and Kinetic Characterization of 85116-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85116-4-RR against Human Caspr2/CNTNAP2 were performed. The affinity constant is below 1 pM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Rattenhirngewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85116-4-RR bindet in WB, IHC, ELISA Caspr2/CNTNAP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Caspr2/CNTNAP2 fusion protein Ag22844
• Vollständiger Name: contactin associated protein-like 2
• Berechnetes Molekulargewicht: 1331 aa, 148 kDa
• Beobachtetes Molekulargewicht: 148 kDa
• GenBank-Zugangsnummer: BC113373
• Gene symbol: Grancalcin
• Gene ID (NCBI): 26047
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Caspr2 is a member of the neurexin family which functions in the vertebrate nervous system as cell adhesion molecules and receptors. This protein, like other neurexin proteins, contains epidermal growth factor repeats and laminin G domains. In addition, it includes an F5/8 type C domain, discoidin/neuropilin- and fibrinogen-like domains, thrombospondin N-terminal-like domains and a putative PDZ binding site. This protein is localized at the juxtaparanodes of myelinated axons, and mediates interactions between neurons and glia during nervous system development and is also involved in localization of potassium channels within differentiating axons. This gene encompasses almost 1.5% of chromosome 7 and is one of the largest genes in the human genome. It is directly bound and regulated by forkhead box protein P2, a transcription factor related to speech and language development.