Cathepsin A Polyklonaler Antikörper

Cathepsin A Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15020-1-PBS

Synonyme

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  WB analysis using 15020-1-AP (same clone as 15020-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15020-1-AP (Cathepsin A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15020-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 15020-1-AP (same clone as 15020-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15020-1-AP (Cathepsin A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15020-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Sp2/0 using 15020-1-AP (same clone as 15020-1-PBS)

  Sp2/0 cells were subjected to SDS PAGE followed by western blot with 15020-1-AP (Cathepsin A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15020-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 15020-1-AP (same clone as 15020-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15020-1-AP (Cathepsin A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15020-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 15020-1-AP (same clone as 15020-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15020-1-AP (Cathepsin A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15020-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 15020-1-AP (same clone as 15020-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15020-1-AP (Cathepsin A antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15020-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 15020-1-AP (same clone as 15020-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15020-1-AP (Cathepsin A antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15020-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat kidney using 15020-1-AP (same clone as 15020-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 15020-1-AP (Cathepsin A antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15020-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat kidney using 15020-1-AP (same clone as 15020-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 15020-1-AP (Cathepsin A antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15020-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

15020-1-PBS bindet in WB, IHC, Indirect ELISA Cathepsin A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cathepsin A fusion protein Ag7073
• Vollständiger Name: cathepsin A
• Berechnetes Molekulargewicht: 54 kDa
• Beobachtetes Molekulargewicht: 54-60, 32, 20 kDa
• GenBank-Zugangsnummer: BC000597
• Gene symbol: Cathepsin A
• Gene ID (NCBI): 5476
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CTSA is also named as PPGB(protective protein for beta-galactosidase), PPCA(protective protein cathepsin A), cathepsin A, carboxypeptidase C, carboxypeptidase L and belongs to the peptidase S10 family. It is a ubiquitously expressed multifunctional enzyme, with deamidase, esterase, and carboxypeptidase activities and a preference for substrates with hydrophobic amino acid residues at the P1-prime position. It can be cleaved into the following 2 chains and defects in CTSA are the cause of galactosialidosis (GSL). This protein has 2 glycosylation sites. CTSA is synthesized as a 54-kDa precursor protein and composed of 32- and 20-kDa subunits linked together by disulfide bonds(PMID: 16461364,19574551).