Cathepsin B Rekombinanter Antikörper

Cathepsin B Rekombinant Antikörper für WB, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

243020A5

Kat-Nr. : 85841-5-RR

Synonyme

Cathepsin B heavy chain, Cathepsin B light chain, Cathepsin B1, Ctsb, EC:3.4.22.1

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  WB analysis using 85841-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85841-5-RR (Cathepsin B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 85841-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85841-5-RR (Cathepsin B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of RAW 264.7 using 85841-5-RR

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using Cathepsin B antibody (85841-5-RR, Clone: 243020A5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of RAW 264.7 using 85841-5-RR

  1x10^6 RAW 264.7 cells were intracellularly stained with 0.25 ug Cathepsin B Recombinant antibody (85841-5-RR, Clone:243020A5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: J774A.1 cells, Maushirngewebe, Mauslebergewebe, RAW 264.7-Zellen
• Erfolgreiche Detektion in IF/ICC: RAW 264.7-Zellen
• Erfolgreiche Detektion in FC (Intra): RAW 264.7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85841-5-RR bindet in WB, IF/ICC, FC (Intra), ELISA Cathepsin B und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Cathepsin B fusion protein Eg3028
• Vollständiger Name: cathepsin B
• Berechnetes Molekulargewicht: 37 kDa
• Beobachtetes Molekulargewicht: 31 kDa, 39 kDa
• GenBank-Zugangsnummer: NM_007798.3
• Gene symbol: Ctsb
• Gene ID (NCBI): 13030
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CTSB(Cathepsin B) is also named as CPSB and belongs to the peptidase C1 family. It participates in intracellular degradation and turnover of proteins. Cathepsin B precursors found in human malignant ascites fluid do not possess mannose-rich carbohydrates suggesting that a defect in the post translational processing of carbohydrate moieties on tumor. Cathepsin B exists as both glycosylated and unglycosylated forms(PMID:1637335). In rat macrophages and hepatocytes pro- cathepsin B is 39 kDa (unglycosylated =35 kDa), whereas in human fibroblasts procathepsin B is 44.5-46kDa (unglycosylated=39kDa)(PMID:2097084). It can be detected the 39 kDa form of pro-CTSB, 31 kDa mature forms in mouse brain by western blot.