Cathepsin D Monoklonaler Antikörper

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• KD/KO Validated

Cathepsin D Monoklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

2F6F7

Kat-Nr. : 66534-1-PBS

Synonyme

CTSD, CPSD, CLN10, Cathepsin D light chain, Cathepsin D heavy chain

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  WB analysis using 66534-1-Ig (same clone as 66534-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66534-1-Ig (Cathepsin D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66534-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 66534-1-Ig (same clone as 66534-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66534-1-Ig (Cathepsin D antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66534-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66534-1-Ig (same clone as 66534-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66534-1-Ig (Cathepsin D antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66534-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66534-1-Ig (same clone as 66534-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66534-1-Ig (Cathepsin D antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66534-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66534-1-Ig (same clone as 66534-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66534-1-Ig (Cathepsin D antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66534-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66534-1-Ig (same clone as 66534-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66534-1-Ig (Cathepsin D antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66534-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66534-1-Ig (same clone as 66534-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66534-1-Ig (Cathepsin D antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66534-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66534-1-Ig (same clone as 66534-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66534-1-Ig (Cathepsin D antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66534-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66534-1-Ig (same clone as 66534-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66534-1-Ig (Cathepsin D antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66534-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66534-1-Ig (same clone as 66534-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Cathepsin D antibody (66534-1-Ig, Clone: 2F6F7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66534-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 66534-1-Ig (same clone as 66534-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Cathepsin D (66534-1-Ig, Clone:2F6F7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66534-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66534-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), ELISA Cathepsin D und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Cathepsin D fusion protein Ag15254
• Vollständiger Name: cathepsin D
• Berechnetes Molekulargewicht: 412 aa, 45 kDa
• Beobachtetes Molekulargewicht: 32 kDa, 48 kDa, 52 kDa
• GenBank-Zugangsnummer: BC016320
• Gene symbol: Cathepsin D
• Gene ID (NCBI): 1509
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CTSD (Cathepsin D) also named CPSD, belongs to the peptidase A1 family. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Human CTSD is synthesized as a 52-kDa precursor that is converted into an active 48-kDa single-chain intermediate in the endosomes, and then into a fully active mature form, composed of a 34-kDa heavy chain and a 14-kDa light chain, in the lysosomes. It is a lysosomal acid protease found in neutrophils and monocytes and involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease (PMID: 27114232, PMID: 30717773, PMID: 30051532).