Cathepsin D Rekombinanter Antikörper

Cathepsin D Rekombinant Antikörper für WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

240775E7

Kat-Nr. : 83743-2-PBS

Synonyme

CTSD, 240775E7, Cathepsin D heavy chain, Cathepsin D light chain, CLN10

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  WB analysis using 83743-2-RR (same clone as 83743-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83743-2-RR (Cathepsin D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83743-2-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 83743-2-RR (same clone as 83743-2-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 83743-2-RR (Cathepsin D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83743-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00695-4

  Sandwich ELISA standard curve of MP00695-4, Human Cathepsin D Recombinant Matched Antibody Pair - PBS only. 83743-6-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1053. 83743-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

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  Sample test of MP00695-4

  Saliva of eight individual healthy human donors was measured. The human Cathepsin D concentration of detected samples was determined to be 139.6 ng/mL with a range of 102.4 - 182.3 ng/mL.

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  Sample test of MP00695-4

  Human milk of eight individual healthy human donors was measured. The human Cathepsin D concentration of detected samples was determined to be 580.4 ng/mL with a range of 270 - 1,071.8 ng/mL.

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  Sample test of MP00695-4

  For the A431 supernatant, the mean human Cathepsin D concentration was determined to be 301.05 ng/mL.

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  Sample test of MP00695-4

  Serum of sixteen individual healthy human donors was measured. The human Cathepsin D concentration of detected samples was determined to be 147.3 ng/mL with a range of 43.2 - 224.2 ng/mL.

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  IF Staining of MCF-7 using 83743-2-RR (same clone as 83743-2-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Cathepsin D antibody (83743-2-RR, Clone: 240775E7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), FLNA antibody (67133-1-Ig, Clone: 1G4H3, red). This data was developed using the same antibody clone with 83743-2-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 83743-2-RR (same clone as 83743-2-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Cathepsin D Recombinant antibody (83743-2-RR, Clone:240775E7) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83743-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

83743-2-PBS bindet in WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Cathepsin D und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Cathepsin D fusion protein Eg1053
• Vollständiger Name: cathepsin D
• Berechnetes Molekulargewicht: 45kDa
• Beobachtetes Molekulargewicht: 32 kDa, 48 kDa, 52 kDa
• GenBank-Zugangsnummer: NM_001909.5
• Gene symbol: Cathepsin D
• Gene ID (NCBI): 1509
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CTSD (Cathepsin D) also named CPSD, belongs to the peptidase A1 family. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Human CTSD is synthesized as a 52-kDa precursor that is converted into an active 48-kDa single-chain intermediate in the endosomes, and then into a fully active mature form, composed of a 34-kDa heavy chain and a 14-kDa light chain, in the lysosomes. It is a lysosomal acid protease found in neutrophils and monocytes and involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease (PMID: 27114232, PMID: 30717773, PMID: 30051532).