Cathepsin D Rekombinanter Antikörper

Cathepsin D Rekombinant Antikörper für WB, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

250329A3

Kat-Nr. : 85964-1-PBS

Synonyme

Cathepsin D heavy chain, Cathepsin D light chain, CLN10, CPSD, CTSD

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  WB analysis using 85964-1-RR (same clone as 85964-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85964-1-RR (Cathepsin D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85964-1-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA standard curve of MP02204-2

  Sandwich ELISA standard curve of MP02204-2, Human Cathepsin D Recombinant Matched Antibody Pair - PBS only. 85964-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2986. 85964-1-PBS was HRP conjugated as the detection antibody. Range: 0.625-40 ng/mL.

• 

  Sample test of MP02204-2

  The mean Cathepsin D concentration was determined to be 137.30 ng/mL in HepG2 cell extract based on a 0.90 mg/mL extract load.

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  Sample test of MP02204-2

  Serum of nine mice was measured. The Cathepsin D concentration of detected samples was determined to be 1,136.75 ng/mL with a range of 773.75-1,505.92 pg/mL.

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Geprüfte Anwendungen

Produktinformation

85964-1-PBS bindet in WB, Sandwich ELISA, Indirect ELISA, Sample test Cathepsin D und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Cathepsin D fusion protein Eg2986
• Vollständiger Name: cathepsin D
• Berechnetes Molekulargewicht: 45 kDa
• Beobachtetes Molekulargewicht: 46 kDa, 28 kDa
• GenBank-Zugangsnummer: NM_009983.3
• Gene symbol: Ctsd
• Gene ID (NCBI): 13033
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CTSD (Cathepsin D) also named CPSD, is a representative aspartic proteinase in lysosomes and is widely distributed in various tissue cells of mammals. The expression level of CTSD varies depending on the tissue, whereas neurons in CNS tissues possess abundant CTSD. CTSD is synthesized as a 52-kDa precursor that is converted into an active ~48-kDa single-chain intermediate in the endosomes, and then into a fully active mature form, composed of a 28-kDa heavy chain and a 14-kDa light chain, in the lysosomes (PMID: 10995834, PMID: 7641679, PMID: 30051532).