Caveolin-1 Monoklonaler Antikörper

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Caveolin-1 Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

6C2B2

Kat-Nr. : 66067-1-PBS

Synonyme

CAV1, Caveolin 1, 6C2B2, BSCL3, CAV

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  WB analysis using 66067-1-Ig (same clone as 66067-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66067-1-Ig (Caveolin-1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66067-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66067-1-Ig (same clone as 66067-1-PBS)

  WB result of Caveolin-1 antibody (66067-1-Ig; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Caveolin-1 transfected HeLa cells. This data was developed using the same antibody clone with 66067-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 66067-1-Ig (same clone as 66067-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66067-1-Ig (Caveolin-1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66067-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66067-1-Ig (same clone as 66067-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66067-1-Ig (Caveolin-1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66067-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66067-1-Ig (same clone as 66067-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66067-1-Ig (Caveolin-1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66067-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66067-1-Ig (same clone as 66067-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66067-1-Ig (Caveolin-1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66067-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66067-1-Ig (same clone as 66067-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66067-1-Ig (Caveolin-1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66067-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66067-1-Ig (same clone as 66067-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66067-1-Ig (Caveolin-1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66067-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66067-1-Ig (same clone as 66067-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66067-1-Ig (Caveolin-1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66067-1-PBS in a different storage buffer formulation.

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  IF Staining of human liver cancer using 66067-1-Ig (same clone as 66067-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Caveolin-1 antibody (66067-1-Ig, Clone: 6C2B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66067-1-PBS in a different storage buffer formulation.

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  IF Staining of human liver cancer using 66067-1-Ig (same clone as 66067-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Caveolin-1 antibody (66067-1-Ig, Clone: 6C2B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66067-1-PBS in a different storage buffer formulation.

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  IF Staining of human skin cancer using 66067-1-Ig (same clone as 66067-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human skin cancer tissue using Caveolin-1 antibody (66067-1-Ig, Clone: 6C2B2 ) at dilution of 1:150 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66067-1-PBS in a different storage buffer formulation.

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  IF Staining of human skin cancer using 66067-1-Ig (same clone as 66067-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human skin cancer tissue using Caveolin-1 antibody (66067-1-Ig, Clone: 6C2B2 ) at dilution of 1:150 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66067-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse kidney using 66067-1-Ig (same clone as 66067-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using Caveolin-1 antibody (66067-1-Ig, Clone: 6C2B2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66067-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 66067-1-Ig (same clone as 66067-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using Caveolin-1 antibody (66067-1-Ig, Clone: 6C2B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66067-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66067-1-PBS bindet in WB, IHC, IF/ICC, IF-P, ELISA Caveolin-1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Caveolin-1 fusion protein Ag8049
• Vollständiger Name: caveolin 1, caveolae protein, 22kDa
• Berechnetes Molekulargewicht: 22 kDa
• Beobachtetes Molekulargewicht: 20-25 kDa
• GenBank-Zugangsnummer: BC006432
• Gene symbol: CAV1
• Gene ID (NCBI): 857
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Caveolin-1 (CAV1), a multifunctional protein, is the main constituent molecule of caveolae and represents a scaffolding molecule for several signaling molecules including epidermal growth factor receptor (PMID: 19641024). Several studies have implicated that a reduced expression of CAV1 was found in cancers including head and neck carcinoma (PMID: 19002186). However, other studies recognize CAV1 as a tumor promoter because CAV1 is overexpressed in various kinds of cancers, especially in oral cancer (PMID: 20558341). Recent study also show that CAV1 is involved in gastric Cancer (PMID: 25339030).