Caveolin-1 Rekombinanter Antikörper

Caveolin-1 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240985E4

Kat-Nr. : 84044-1-PBS

Synonyme

Caveolin 1, CAV1, CGL3, CAV, BSCL3

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  WB analysis using 84044-1-RR (same clone as 84044-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84044-1-RR (Caveolin-1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84044-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 84044-1-RR (same clone as 84044-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 84044-1-RR (Caveolin-1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84044-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 84044-1-RR (same clone as 84044-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84044-1-RR (Caveolin-1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84044-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung squamous cell carcinoma using 84044-1-RR (same clone as 84044-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue slide using 84044-1-RR (Caveolin-1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84044-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84044-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84044-1-RR against Human Caveolin-1 were performed. The affinity constant is 1.53 nM.

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Geprüfte Anwendungen

Produktinformation

84044-1-PBS bindet in WB, IHC, ELISA Caveolin-1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Caveolin-1 fusion protein Ag8049
• Vollständiger Name: caveolin 1, caveolae protein, 22kDa
• Berechnetes Molekulargewicht: 22 kDa
• Beobachtetes Molekulargewicht: 20-25 kDa
• GenBank-Zugangsnummer: BC006432
• Gene symbol: CAV1
• Gene ID (NCBI): 857
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Caveolin-1 (CAV1), a multifunctional protein, is the main constituent molecule of caveolae and represents a scaffolding molecule for several signaling molecules including epidermal growth factor receptor (PMID: 19641024). Several studies have implicated that a reduced expression of CAV1 was found in cancers including head and neck carcinoma (PMID: 19002186). However, other studies recognize CAV1 as a tumor promoter because CAV1 is overexpressed in various kinds of cancers, especially in oral cancer (PMID: 20558341). Recent study also show that CAV1 is involved in gastric Cancer (PMID: 25339030).