Caveolin-3 Polyklonaler Antikörper

Caveolin-3 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 28358-1-AP

Synonyme

CAV3, Caveolin3, M-caveolin, LQT9, LGMD1C

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  WB analysis of mouse colon using 28358-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 28358-1-AP (Caveolin-3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 28358-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 28358-1-AP (Caveolin-3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 28358-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 28358-1-AP (Caveolin-3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse heart using 28358-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 28358-1-AP (Caveolin-3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 28358-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 28358-1-AP (Caveolin-3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skeletal muscle using 28358-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 28358-1-AP (Caveolin-3 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skeletal muscle using 28358-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 28358-1-AP (Caveolin-3 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 28358-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 28358-1-AP (Caveolin-3 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 28358-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 28358-1-AP (Caveolin-3 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse skeletal muscle using 28358-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using Caveolin-3 antibody (28358-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse skeletal muscle using 28358-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using Caveolin-3 antibody (28358-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of H9C2 using 28358-1-AP

  Immunofluorescent analysis of (4% PFA) fixed H9C2 cells using Caveolin-3 antibody (28358-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Kolongewebe, Mausherzgewebe, Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: Mausherzgewebe, Maus-Skelettmuskelgewebe, Ratten-Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IF/ICC: H9C2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

28358-1-AP bindet in WB, IHC, IF/ICC, IF-P, ELISA Caveolin-3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Caveolin-3 fusion protein Ag28009
• Vollständiger Name: caveolin 3
• Berechnetes Molekulargewicht: 151 aa, 17 kDa
• Beobachtetes Molekulargewicht: 20 kDa
• GenBank-Zugangsnummer: BC069368
• Gene symbol: Caveolin-3
• Gene ID (NCBI): 859
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human,mouse	WB	Oxid Med Cell Longev; Trophoblasts Modulate the Ca2+ Oscillation and Contraction of Myometrial Smooth Muscle Cells by Small Extracellular Vesicle- (sEV-) Mediated Exporting of miR-25-3p during Premature Labor.; Authors - Lin Wang
mouse	WB	Cell Metab; Muscle-derived small extracellular vesicles induce liver fibrosis during overtraining; Authors - Ya Liu
rat	WB,IF	Int Heart J; Decrease in Caveolae-Gαq Interaction Mediates Pressure Overload-Induced Cardiac Remodeling in Rats; Authors - Lijin Wang