MCP-1/CCL2 Rekombinanter Antikörper

MCP-1/CCL2 Rekombinant Antikörper für WB, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Ratte

Anwendung

WB, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

240341G3

Kat-Nr. : 83370-2-PBS

Synonyme

Ccl2, 240341G3, C-C motif chemokine 2, CCL 2, CCL-2

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  WB analysis of NR8383 using 83370-2-RR (same clone as 83370-2-PBS)

  Untreated, LPS and Brefeldin A treated NR8383 cells were subjected to SDS PAGE followed by western blot with 83370-2-RR (Ccl2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83370-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00390-3

  Sandwich ELISA standard curve of MP00390-3, rat MCP-1/CCL-2 Recombinant Matched Antibody Pair - PBS only. 83370-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard: Eg0397. 83370-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL..

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  Sample test of MP00390-3

  Serum of sixteen rat donors was measured. The MCP-1/CCL-2 concentration of detected samples was determined to be 8,981.3 pg/mL with a range of 5,770.2 - 16,068.9 pg/mL.

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  Sample test of MP00390-3

  Rat spleen cell cultures (1/2 spleen; 1-2 mm pieces in 50 mL DMEM supplemented with 10% fetal bovine serum, containing 100 ng/mL LPS) were collected after culturing for 3 days in a CO2-enriched (9.5%) incubator. The cell culture supernatant was assayed for rat MCP-1/CCL2 and measured 5,804.62 pg/mL.

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  Affinity and Kinetic Characterization of 83370-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83370-2-RR against Rat MCP-1 were performed. The affinity constant is 0.836 nM.

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Geprüfte Anwendungen

Produktinformation

83370-2-PBS bindet in WB, Sandwich ELISA, Indirect ELISA, Sample test MCP-1/CCL2 und zeigt Reaktivität mit Ratten

• Getestete Reaktivität: Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MCP-1/CCL2 fusion protein Eg0397
• Vollständiger Name: chemokine (C-C motif) ligand 2
• Berechnetes Molekulargewicht: 16 kDa
• Beobachtetes Molekulargewicht: 20 kDa
• GenBank-Zugangsnummer: NM_031530
• Gene symbol: MCP-1/CCL2
• Gene ID (NCBI): 24770
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Monocyte chemoattractant protein-1 (MCP-1/CCL2) is one of the key chemokines that regulate migration and infiltration of monocytes/macrophages. Both CCL2 and its receptor CCR2 have been demonstrated to be induced and involved in various diseases. Migration of monocytes from the blood stream across the vascular endothelium is required for routine immunological surveillance of tissues, as well as in response to inflammation.