CD74 Rekombinanter Antikörper

CD74 Rekombinant Antikörper für IHC, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

IHC, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250109B11

Kat-Nr. : 85802-2-PBS

Synonyme

Class-II-associated invariant chain peptide, CLIP, H-2 class II histocompatibility antigen gamma chain, Ia antigen-associated invariant chain, Ii

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  Sandwich ELISA sample test of MP02137-1

  RAW 264.7 were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for mouse CD74, and measured 14.17 ng/mL.

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  Sandwich ELISA sample test of MP02137-1

  The mean CD74 concentration was determined to be 856.01 ng/mL in mouse thymus tissue extract based on a 5.80 mg/mL extract load and 789.39 ng/mL in mouse spleen tissue extract based on a 8.50 mg/mL extract load.

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  Sandwich ELISA sample test of MP02137-1

  Serum of sixteen mice was measured. The CD74 concentration of detected samples was determined to be 21.51 pg/mL with a range of 12.61-42.94 pg/mL.

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  Sandwich ELISA standard curve of MP02137-1

  Sandwich ELISA standard curve of MP02137-1, Mouse CD74 Recombinant Matched Antibody Pair - PBS only. 85802-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2716. 85802-2-PBS was HRP conjugated as the detection antibody. Range: 0.391-25 ng/mL.

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  IHC staining of mouse spleen using 85802-2-RR (same clone as 85802-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85802-2-RR (CD74 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85802-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 85802-2-RR (same clone as 85802-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85802-2-RR (CD74 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85802-2-PBS in a different storage buffer formulation.

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  IF Staining of mouse spleen using 85802-2-RR (same clone as 85802-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD74 antibody (85802-2-RR, Clone: 250109B11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85802-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP02137-1

  Cytometric bead array standard curve of MP02137-1, MOUSE CD74 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85802-1-PBS. Detection antibody: 85802-2-PBS. Standard: Eg2716. Range: 0.781-50 ng/mL.

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Geprüfte Anwendungen

Produktinformation

85802-2-PBS bindet in IHC, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA CD74 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD74 fusion protein Eg2716
• Vollständiger Name: CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated)
• Berechnetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: NM_001042605.1
• Gene symbol: Cd74
• Gene ID (NCBI): 16149
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD74, also known as MHC class II invariant chain Ii or HLA-DR-associated invariant chain, is a non-polymorphic type II transmembrane glycoprotein. CD74 is mainly distributed in B cells, monocytes, Langerhans cells, dendritic cells and thymic epithelial cells, and is also expressed in epithelial cells. CD74 plays a key role in MHC class II antigen processing and also serves as a cell surface receptor for the macrophage cytokine MIF. CD74 plays an important role in cardiovascular diseases such as atherosclerosis, ischemic heart disease, and myocardial hypertrophy.(PMID: 15475450; PMID: 27752708; PMID: 38992165; PMID: 36712241).