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  • Featured Product
  • KD/KO Validated

Cdc20 Polyklonaler Antikörper

Cdc20 Polyklonal Antikörper für IF/ICC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Kat-Nr. : CL488-10252

Synonyme

bA276H19.3, CDC20, CDC20A, p55CDC

Filter:
  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated Cdc20 Polyclonal ant (CL488-10252)

    IF Staining of HeLa using CL488-10252

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 Cdc20 antibody (CL488-10252) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using CoraLite® Plus 488-conjugated Cdc20 Polyclonal ant (CL488-10252)

    IF Staining of U2OS using CL488-10252

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CoraLite® Plus 488 Cdc20 antibody (CL488-10252) at dilution of 1:200.

Geprüfte Anwendungen

Erfolgreiche Detektion in IF/ICCHeLa-Zellen, U2OS-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-10252 bindet in IF/ICC Cdc20 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Cdc20 fusion protein Ag0295
Vollständiger Name cell division cycle 20 homolog (S. cerevisiae)
Berechnetes Molekulargewicht 55 kDa
Beobachtetes Molekulargewicht 55 kDa
GenBank-ZugangsnummerBC001088
Gene symbol CDC20
Gene ID (NCBI) 991
Konjugation CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cdc20 (Cell-division cycle protein 20 homologue) is also named as p55CDC and belongs to the WD repeat CDC20/Fizzy family. Cdc20 has important functions in chromosome segregation and mitotic exit. Cdc20 is the target of the spindle assembly checkpoint (SAC) and a key cofactor of the anaphase-promoting complex or cyclosome (APC/CCDC20) E3 ubiquitin ligase, thus regulating APC/C ubiquitin activity on specific substrates for their subsequent degradation by the proteasome (PMID: 28202332). The 55-kDa band corresponded to the size of Cdc20 (PMID: 22566641).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
IF protocol for CL Plus 488 Cdc20 antibody CL488-10252Protokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen