p21 Polyklonaler Antikörper

p21 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(93)

Rezensionen (1)

Kat-Nr. : 28248-1-AP

Synonyme

Cdkn1a, Cdkn1, P21 protein, p21Cip1, p21WAF

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  WB analysis using 28248-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28248-1-AP (p21 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 28248-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28248-1-AP (p21 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of NIH/3T3 using 28248-1-AP

  IP result of anti-p21 (IP:28248-1-AP, 4ug; Detection:28248-1-AP 1:1000) with NIH/3T3 cells lysate 1920 ug.

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  IF Staining of NIH/3T3 using 28248-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using 28248-1-AP (p21 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). F-actin is stained using CL555-phalloidin (red).

• 

  IF Staining of NIH/3T3 using 28248-1-AP

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using p21 antibody (28248-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-phalloidin (green).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-12-Zellen, DC2.4-Zellen, Neuro-2a-Zellen, NIH/3T3-Zellen, RAW 264.7-Zellen, Sp2/0-Zellen
• Erfolgreiche IP: NIH/3T3-Zellen
• Erfolgreiche Detektion in IF/ICC: NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 76 publications below
• IHC: See 16 publications below
• IF: See 20 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

28248-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA p21 und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: p21 fusion protein Ag28394
• Vollständiger Name: cyclin-dependent kinase inhibitor 1A (P21)
• Berechnetes Molekulargewicht: 18 kDa
• Beobachtetes Molekulargewicht: 18 kDa
• GenBank-Zugangsnummer: NM_001111099
• Gene symbol: p21
• Gene ID (NCBI): 12575
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CDKN1A (p21, CIP1, WAF1) is a cyclin-dependent kinase inhibitor. CDKN1A binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at the G1 phase. The expression of CDKN1A is induced by wild-type but not mutant p53 protein, through which CDKN1A mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. CDKN1A can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. CDKN1A was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation.

Publikationen

Species	Application	Title
mouse	WB	Bioact Mater; Chemo-immunotherapy by dual-enzyme responsive peptide self-assembling abolish melanoma; Authors - Yuhan Wang
mouse	WB	Redox Biol; Deficiency of S100 calcium binding protein A9 attenuates vascular dysfunction in aged mice; Authors - Boying Zhao
mouse	IF	EMBO J; Kinesin-like motor protein KIF23 maintains neural stem and progenitor cell pools in the developing cortex; Authors - Sharmin Naher
mouse	IF	Dev Cell; Endoderm development requires centrioles to restrain p53-mediated apoptosis in the absence of ERK activity.; Authors - Chang Xie
mouse	WB	Aging Cell; Excessive processing and acetylation of OPA1 aggravate age-related hearing loss via the dysregulation of mitochondrial dynamics; Authors - Andi Zhang
mouse	WB	Biomed Pharmacother; Seno-antigen-pulsed dendritic cell vaccine induce anti-aging immunity to improve adipose tissue senescence and metabolic abnormalities; Authors - Yin Cao

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Bárbara (Verified Customer) (07-31-2025)

We used the p21 rabbit antibody from Proteintech for immunofluorescence on mouse brain, followed by confocal microscopy. The antibody produced a strong, specific signal . Minimal background and high signal-to-noise ratio made it easy to interpret.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/250
• Cell Tissue Type: Brain

p21 Antibody Immunofluorescence validation (1/250 dilution) in Brain (Cat no:28248-1-AP)