Ceruloplasmin Monoklonaler Antikörper

Ceruloplasmin Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3C8A5

Kat-Nr. : 66156-1-PBS

Synonyme

Ceruloplasmin, ceruloplasmin (ferroxidase), CP, CP 2, Ferroxidase

---

• 

  WB analysis of rat liver using 66156-1-Ig (same clone as 66156-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66156-1-Ig (Ceruloplasmin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66156-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 66156-1-Ig (same clone as 66156-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66156-1-Ig (Ceruloplasmin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66156-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HSC-T6 using 66156-1-Ig (same clone as 66156-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66156-1-Ig (Ceruloplasmin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66156-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human kidney using 66156-1-Ig (same clone as 66156-1-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 66156-1-Ig (Ceruloplasmin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66156-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human blood using 66156-1-Ig (same clone as 66156-1-PBS)

  human blood was subjected to SDS PAGE followed by western blot with 66156-1-Ig (Ceruloplasmin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66156-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 66156-1-Ig (same clone as 66156-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66156-1-Ig (Ceruloplasmin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66156-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 66156-1-Ig (same clone as 66156-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66156-1-Ig (Ceruloplasmin antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66156-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66156-1-Ig (same clone as 66156-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66156-1-Ig (Ceruloplasmin antibody at dilution of 1:50. This data was developed using the same antibody clone with 66156-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66156-1-Ig (same clone as 66156-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66156-1-Ig (Ceruloplasmin antibody at dilution of 1:50. This data was developed using the same antibody clone with 66156-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 66156-1-Ig (same clone as 66156-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66156-1-Ig (Ceruloplasmin antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66156-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 66156-1-Ig (same clone as 66156-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66156-1-Ig (Ceruloplasmin antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66156-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 66156-1-Ig (same clone as 66156-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66156-1-Ig (Ceruloplasmin antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66156-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 66156-1-Ig (same clone as 66156-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66156-1-Ig (Ceruloplasmin antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66156-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66156-1-Ig (same clone as 66156-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Ceruloplasmin antibody (66156-1-Ig, Clone: 3C8A5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66156-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

66156-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA Ceruloplasmin und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Ceruloplasmin fusion protein Ag15535
• Vollständiger Name: ceruloplasmin (ferroxidase)
• Berechnetes Molekulargewicht: 1065 aa, 122 kDa
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: BC146663
• Gene symbol: Ceruloplasmin
• Gene ID (NCBI): 1356
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ceruloplasmin (CP) is a serum glycoprotein synthesized by hepatocytes and functions as the major transport protein of copper in blood where it acts as ferrodoxidase and is required for iron transport of transferrin. The intact ceruloplasmin migrated around 130-140 kDa and some proteolytic cleaved fragments could also be observed.