Chromogranin A Rekombinanter Antikörper

Chromogranin A Rekombinant Antikörper für WB, IHC, IF-P, Cytometric bead array, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

250318B12

Kat-Nr. : 86447-1-PBS

Synonyme

Beta-granin, Catestatin, CgA, Chga, Chromogranin-A

---

• 

  WB analysis of PC-12 using 86447-1-RR (same clone as 86447-1-PBS)

  PC-12 cells were subjected to SDS PAGE followed by western blot with 86447-1-RR (Chga antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86447-1-PBS in a different storage buffer formulation.

• 

  Sample test of MP02444-1

  The mean Chromogranin a concentration was determined to be 77.6 ng/mL in mouse serum.

• 

  IHC staining of mouse pancreas using 86447-1-RR (same clone as 86447-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 86447-1-RR (Chromogranin A antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86447-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse pancreas using 86447-1-RR (same clone as 86447-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using Chromogranin A antibody (86447-1-RR, Clone: 250318B12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86447-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP02444-1

  Cytometric bead array standard curve of MP02444-1, MOUSE Chromogranin a Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86447-1-PBS. Detection antibody: 86447-2-PBS. Standard: Eg2760. Range: 0.625-80 ng/mL.

---

Geprüfte Anwendungen

Produktinformation

86447-1-PBS bindet in WB, IHC, IF-P, Cytometric bead array, Indirect ELISA, Sample test Chromogranin A und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Chromogranin A fusion protein Eg2760
• Vollständiger Name: chromogranin A
• Berechnetes Molekulargewicht: 52 kDa
• Beobachtetes Molekulargewicht: 70-85 kDa
• GenBank-Zugangsnummer: NM_007693.2
• Gene symbol: Chga
• Gene ID (NCBI): 12652
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Chromogranin A is a member of the granin family of neuroendocrine secretory proteins. It is located in secretory vesicles of neurons and endocrine cells. Chromogranin A is the precursor to several functional peptides including vasostatin, pancreastatin, catestatin and parastatin. These peptides negatively modulate the neuroendocrine function of the releasing cell (autocrine) or nearby cells (paracrine). CgA is one of the most used tumor markers in NET's (neuroendocrine tumors) , and elevated CgA concentrations have been demonstrated in serum or plasma of patients with different types of these tumors.