Chicken IgY Monoklonaler Antikörper

Chicken IgY Monoklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Huhn

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1E12G2

Kat-Nr. : 67405-1-PBS

Synonyme

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  WB analysis of Recombinant protein using 67405-1-Ig (same clone as 67405-1-PBS)

  100 nL chicken egg were subjected to SDS-PAGE at non-reducing condition followed by western blot with 67405-1-Ig (chicken IgY antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. . This data was developed using the same antibody clone with 67405-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Recombinant protein using 67405-1-Ig (same clone as 67405-1-PBS)

  50 nL chicken egg were subjected to SDS-PAGE at non-reducing condition followed by western blot with 67405-1-Ig (chicken IgY antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. . This data was developed using the same antibody clone with 67405-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Recombinant protein using 67405-1-Ig (same clone as 67405-1-PBS)

  25 nL chicken egg were subjected to SDS-PAGE at non-reducing condition followed by western blot with 67405-1-Ig (chicken IgY antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. . This data was developed using the same antibody clone with 67405-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Recombinant protein using 67405-1-Ig (same clone as 67405-1-PBS)

  12.5 nL chicken egg were subjected to SDS-PAGE at non-reducing condition followed by western blot with 67405-1-Ig (chicken IgY antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. . This data was developed using the same antibody clone with 67405-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Recombinant protein using 67405-1-Ig (same clone as 67405-1-PBS)

  12.5 nL chicken egg were subjected to SDS-PAGE under reducing condition followed by western blot with 67405-1-Ig (chicken IgY antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67405-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67405-1-PBS bindet in WB, Indirect ELISA Chicken IgY und zeigt Reaktivität mit Huhn

• Getestete Reaktivität: Huhn
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: Chicken IgY
• Beobachtetes Molekulargewicht: 180 kDa, 70 kDa
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IgY (immunoglobulin Y) is a type of immunoglobulin found in birds and reptiles. High concentration of IgY exists in chicken egg yolk. In chickens, IgY is functional same to IgG. IgY antibodies do not cross-react with Fc receptors, mammalian immunoglobulins such as rheumatoid factors and HAMA or activate the complement system thus could reduce false positive results in immunological assays. This antibody was generated from chicken IgY that extracted from chicken eggs. This product recognizes the heavry chain of checken IgY and can detect the contact form of chicken IgY (~180 kDa) and reduced form (~70 kDa).