Chk1 Polyklonaler Antikörper

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• KD/KO Validated

Chk1 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25887-1-PBS

Synonyme

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  WB analysis using 25887-1-AP (same clone as 25887-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 25887-1-AP (Chk1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25887-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293T using 25887-1-AP (same clone as 25887-1-PBS)

  IP result of anti-Chk1 (IP:25887-1-AP, 4ug; Detection:25887-1-AP 1:600) with HEK-293T cells lysate 4000ug. This data was developed using the same antibody clone with 25887-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 25887-1-AP (same clone as 25887-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25887-1-AP (CHK1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 25887-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 25887-1-AP (same clone as 25887-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25887-1-AP (CHK1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 25887-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293T using 25887-1-AP (same clone as 25887-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293T cells using Chk1 antibody (25887-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25887-1-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293T using 25887-1-AP (same clone as 25887-1-PBS)

  1X10^6 HEK-293T cells were intracellularly stained with 0.2 ug Anti-Human Chk1 (25887-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 25887-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

25887-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA Chk1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Chk1 fusion protein Ag22993
• Vollständiger Name: CHK1 checkpoint homolog (S. pombe)
• Berechnetes Molekulargewicht: 54 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC004202
• Gene symbol: Chk1
• Gene ID (NCBI): 1111
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CHEK1(Checkpoint kinase-1) is also named as CHK1 and belongs to the protein kinase superfamily. It is implicated in a circuit in which it activates checkpoints, DNA repair and proliferating cell nuclear antigen and FANCD2 monoubiquitinylation(PMID:21389083). CHEK1 protects vertebrate cells against spontaneous chromosome missegregation and is required to sustain anaphase delay when spindle function is disrupted by taxol(PMID:17276342). It has 3 isoforms produced by alternative splicing with the molecular mass of 54 kDa, 44 kDa and 50 kDa.