Chk1 Monoklonaler Antikörper

Chk1 Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3A3A5

Kat-Nr. : 60277-1-PBS

Synonyme

CHEK1, Chk1

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  WB analysis using 60277-1-Ig (same clone as 60277-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60277-1-Ig (Chk1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60277-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 60277-1-Ig (same clone as 60277-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60277-1-Ig (Chk1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60277-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60277-1-Ig (same clone as 60277-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60277-1-Ig (Chk1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60277-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60277-1-Ig (same clone as 60277-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60277-1-Ig (Chk1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60277-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

60277-1-PBS bindet in WB, IHC, Indirect ELISA Chk1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Chk1 fusion protein Ag0409
• Vollständiger Name: CHK1 checkpoint homolog (S. pombe)
• Berechnetes Molekulargewicht: 54 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC004202
• Gene symbol: Chk1
• Gene ID (NCBI): 1111
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CHEK1(Checkpoint kinase-1) is also named as CHK1 and belongs to the protein kinase superfamily. It is implicated in a circuit in which it activates checkpoints, DNA repair and proliferating cell nuclear antigen and FANCD2 monoubiquitinylation(PMID:21389083). CHEK1 protects vertebrate cells against spontaneous chromosome missegregation and is required to sustain anaphase delay when spindle function is disrupted by taxol(PMID:17276342). It has 3 isoforms produced by alternative splicing with the molecular mass of 54 kDa, 44 kDa and 50 kDa.