Chromogranin A Rekombinanter Antikörper

Chromogranin A Rekombinant Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

243048H7

Kat-Nr. : 85605-2-RR

Synonyme

CGA, CHGA, AL-11, Chromogranin-A, EA-92

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  WB analysis using 85605-2-RR

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 85605-2-RR (CHGA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 85605-2-RR

  TT cells were subjected to SDS PAGE followed by western blot with 85605-2-RR (CHGA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 85605-2-RR

  PC-12 cells were subjected to SDS PAGE followed by western blot with 85605-2-RR (CHGA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 85605-2-RR

  Pig adrenal gland tissue was subjected to SDS PAGE followed by western blot with 85605-2-RR (CHGA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse pancreas using 85605-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 85605-2-RR (Chromogranin A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendix using 85605-2-RR

  Immunohistochemical analysis of paraffin-embedded human appendix tissue slide using 85605-2-RR (Chromogranin A antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

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  IHC staining of mouse colon using 85605-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 85605-2-RR (Chromogranin A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 85605-2-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 85605-2-RR (Chromogranin A antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas using 85605-2-RR

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 85605-2-RR (CHGA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 85605-2-RR

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 85605-2-RR (CHGA antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse pancreas using 85605-2-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using Chromogranin A antibody (85605-2-RR, Clone: 243048H7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse small intestine using 85605-2-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using Chromogranin A antibody (85605-2-RR, Clone: 243048H7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 85605-2-RR

  Biolayer interferometry (BLl) kinetic assays of 85605-2-RR against Human Chromogranin A were performed. The affinity constant is 3.39 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen, PC-12-Zellen, Hausschwein-Nebennierengewebe
• Erfolgreiche Detektion in IHC: humanes Pankreasgewebe, humanes Appendix-Gewebe, humanes Kolongewebe, Maus-Kolongewebe, Maus-Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maus-Dünndarmgewebe, Maus-Pankreasgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85605-2-RR bindet in WB, IHC, IF-P, ELISA Chromogranin A und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Chromogranin A fusion protein Ag19780
• Vollständiger Name: chromogranin A (parathyroid secretory protein 1)
• Berechnetes Molekulargewicht: 51 kDa
• Beobachtetes Molekulargewicht: 70-85 kDa
• GenBank-Zugangsnummer: BC006459
• Gene symbol: Chromogranin A
• Gene ID (NCBI): 1113
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Chromogranin A is a member of the granin family of neuroendocrine secretory proteins. It is located in secretory vesicles of neurons and endocrine cells. Chromogranin A is the precursor to several functional peptides including vasostatin, pancreastatin, catestatin and parastatin. These peptides negatively modulate the neuroendocrine function of the releasing cell (autocrine) or nearby cells (paracrine). CgA is one of the most used tumor markers in NET's (neuroendocrine tumors) , and elevated CgA concentrations have been demonstrated in serum or plasma of patients with different types of these tumors.