Chromogranin B Polyklonaler Antikörper

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Chromogranin B Polyklonal Antikörper für WB, IHC, IF-P, IF-Fro, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, IF-Fro, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14968-1-PBS

Synonyme

CgB, CHGB, SCG1, Chromogranin-B, PE-11

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  WB analysis of PC-12 using 14968-1-AP (same clone as 14968-1-PBS)

  PC-12 cells were subjected to SDS PAGE followed by western blot with 14968-1-AP (Chromogranin B antibody at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14968-1-PBS in a different storage buffer formulation.

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  WB analysis using 14968-1-AP (same clone as 14968-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14968-1-AP (Chromogranin B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14968-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 14968-1-AP (same clone as 14968-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14968-1-AP (Chromogranin B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14968-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 14968-1-AP (same clone as 14968-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 14968-1-AP (Chromogranin B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14968-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas using 14968-1-AP (same clone as 14968-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14968-1-AP (Chromogranin B antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 14968-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas using 14968-1-AP (same clone as 14968-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14968-1-AP (Chromogranin B antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 14968-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 14968-1-AP (same clone as 14968-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 14968-1-AP (Chromogranin B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14968-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse pancreas using 14968-1-AP (same clone as 14968-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 14968-1-AP (Chromogranin B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14968-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse colon using 14968-1-AP (same clone as 14968-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using Chromogranin B antibody (14968-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14968-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse colon using 14968-1-AP (same clone as 14968-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using Chromogranin B antibody (14968-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14968-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse colon using 14968-1-AP (same clone as 14968-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse colon tissue using 14968-1-AP (Chromogranin B antibody) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14968-1-PBS in a different storage buffer formulation.

• 

  FC experiment of PC-12 using 14968-1-AP (same clone as 14968-1-PBS)

  1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human Chromogranin B (14968-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 14968-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

14968-1-PBS bindet in WB, IHC, IF-P, IF-Fro, FC (Intra), Indirect ELISA Chromogranin B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Chromogranin B fusion protein Ag6833
• Vollständiger Name: chromogranin B (secretogranin 1)
• Berechnetes Molekulargewicht: 78 kDa
• Beobachtetes Molekulargewicht: 70-100 kDa
• GenBank-Zugangsnummer: BC000375
• Gene symbol: Chromogranin B
• Gene ID (NCBI): 1114
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Chromogranin B is also named as Secretogranin-1, SgI, CgB, CHGB and SCG1. CHGB, a secretory granule protein, inserts itself into membrane and forms a chloride-conducting channel (PMID: 30456382). Native CHGB distributes nearly equally in soluble and membrane-bound forms, and both reconstitute highly selective anion channels in membrane (PMID: 37435575). The calculated molecular weight of Chromogranin B is at 78kDa. It has been shown that CHGB has a clear degradation band at ~50 kDa (https://www.biorxiv.org/content/10.1101/2019.12.28.890053v1.full.pdf).