Citrate synthase Rekombinanter Antikörper

Citrate synthase Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

251433D2

Kat-Nr. : 86549-1-RR

Synonyme

CS, CISY, Citrate, Citrate synthase, mitochondrial, EC:2.3.3.1

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  WB analysis using 86549-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86549-1-RR (CS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 86549-1-RR

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 86549-1-RR (CS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 86549-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CS antibody (86549-1-RR, Clone: 251433D2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of U2OS using 86549-1-RR

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CS antibody (86549-1-RR, Clone: 251433D2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 86549-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86549-1-RR against Human Citrate synthase were performed. The affinity constant is 0.995 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, Mausherzgewebe, NIH/3T3-Zellen
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86549-1-RR bindet in WB, IF/ICC, ELISA Citrate synthase und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Citrate synthase fusion protein Ag9117
• Vollständiger Name: citrate synthase
• Berechnetes Molekulargewicht: 466 aa, 52 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: BC010106
• Gene symbol: Citrate Synthase
• Gene ID (NCBI): 1431
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Citrate synthase (CS), the first and rate-limiting enzyme of the tricarboxylic acid cycle, plays a key role in regulating energy generation of mitochondrial respiration(PMID:19479947).It belongs to the citrate synthase family. The deduced 466-amino acid protein contains an N-terminal mitochondrial targeting sequence and a motif highly conserved in citrate synthases(PMID:12549038). It can exsit as a dimer(PMID:8749851). Northern blot analysis detected no CS expression in thymus and small intestine(PMID:12549038).