Claudin 1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Claudin 1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (3)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(161)

Kat-Nr. : 28674-1-AP

Synonyme

Caudin 1, CLDN1, Claudin-1, CLD1, ILVASC

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  WB analysis of A431 using 28674-1-AP

  WB result of Claudin 1 antibody (28674-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Claudin 1 transfected A431 cells.

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  WB analysis using 28674-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28674-1-AP (Claudin 1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 28674-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28674-1-AP (Claudin 1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 28674-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28674-1-AP (Claudin 1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 28674-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28674-1-AP (Claudin 1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 28674-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 28674-1-AP (Claudin 1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 28674-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 28674-1-AP (Claudin 1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skin using 28674-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 28674-1-AP (Claudin 1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skin using 28674-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 28674-1-AP (Claudin 1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 28674-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 28674-1-AP (Claudin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 28674-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 28674-1-AP (Claudin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HaCaT using 28674-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using Claudin 1 antibody (28674-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of HUVEC using 28674-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using Claudin 1 antibody (28674-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HepG2-Zellen, Maushautgewebe, Rattenhautgewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Hautkrebsgewebe, Maus-Kolongewebe, Maushautgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HaCaT-Zellen, HUVEC-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 116 publications below
• IHC: See 33 publications below
• IF: See 40 publications below

Produktinformation

28674-1-AP bindet in WB, IHC, IF/ICC, ELISA Claudin 1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Hund, Maus, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Claudin 1 fusion protein Ag29269
• Vollständiger Name: claudin 1
• Berechnetes Molekulargewicht: 211 aa, 23 kDa
• Beobachtetes Molekulargewicht: 20-23 kDa
• GenBank-Zugangsnummer: BC012471
• Gene symbol: Claudin 1
• Gene ID (NCBI): 9076
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Claudins are a family of proteins that are the most important components of tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. 23 claudins have been identified. They are small (20-27 kilodalton (kDa)) proteins with similar structures. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm. Claudin-1 is an integral membrane protein expressed primarily in keratinocytes and normal mammary epithelial cells. Claudin 1 forms tight junctions with other claudin proteins and plays an important role in the intestinal epithelial barrier.

Publikationen

Species	Application	Title
	IF	Microbiome; Arula-7 powder improves diarrhea and intestinal epithelial tight junction function associated with its regulation of intestinal flora in calves infected with pathogenic Escherichia coli O1; Authors - Hao Chen
mouse	WB	J Nanobiotechnology; Human umbilical cord mesenchymal stem cells derived exosome shuttling mir-129-5p attenuates inflammatory bowel disease by inhibiting ferroptosis; Authors - Zhiping Wei
mouse	IF	Matrix Biol; Ameloblastin promotes polarization of ameloblast cell lines in a 3-D cell culture system; Authors - Gayathri Visakan
human	WB	Int J Biol Macromol; Anionic polysaccharides benefit the bioavailability of pork myofibrillar protein gels: Evidence from a perspective of protein absorption and metabolism; Authors - Xia Yu
rat	WB,IF	Phytomedicine; Licorice-regulated gut-joint axis for alleviating collagen-induced rheumatoid arthritis; Authors - Di Yang
mouse,human	WB	Biomed Pharmacother; Bacterial butyrate mediates the anti-atherosclerotic effect of silybin; Authors - Hao-Ran Shen