Claudin 2 Polyklonaler Antikörper

Claudin 2 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (3)

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(15)

Kat-Nr. : 26912-1-AP

Synonyme

CLDN2, SP82, PSEC0059

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  WB analysis using 26912-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26912-1-AP (Claudin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 26912-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26912-1-AP (Claudin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, Caco-2-Zellen, HEK-293-Zellen, HepG2-Zellen, HT-29-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 10 publications below
• IF: See 6 publications below

Produktinformation

26912-1-AP bindet in WB, IF, ELISA Claudin 2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Huhn, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Claudin 2 fusion protein Ag25527
• Vollständiger Name: claudin 2
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: BC014424
• Gene symbol: Claudin 2
• Gene ID (NCBI): 9075
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Claudin 2, also named as SP82, belongs to the claudin family. Claudin 2 is a tetraspan membrane protein consisting of two extracellular domains (ECL1 and ECL2), a small intracellular loop connecting the second and third transmembrane sections and short intracellular N and C terminal portions. Claudin 2 is a cation-selective channel forming TJ protein expressed in leaky epithelia. Claudin 2 is a 230 amino acid transmembrane protein, with a calculated molecular mass of 25 kDa (PMID: 31726679)

Publikationen

Species	Application	Title
mouse	IF	J Ethnopharmacol; The rhizomes of Atractylodes macrocephala Koidz improve gastrointestinal health and pregnancy outcomes in pregnant mice via modulating intestinal barrier and water-fluid metabolism; Authors - Wang Chenxing
human	WB	Bioconjug Chem; Transforms of Cell Surface Glycoproteins Charge Influences Tumor Cell Metastasis via Atypically Inhibiting Epithelial-Mesenchymal Transition Including Matrix Metalloproteinases and Cell Junctions; Authors - Mingzhe Wang
rat	WB	Pharmaceuticals (Basel); Exploring the Underlying Mechanism of Weiling Decoction Alleviates Cold-Dampness Diarrhea Based on Network Pharmacology, Transcriptomics, Molecular Docking and Experimental Validation; Authors - Yannan Zhang
mouse	WB	Naunyn Schmiedebergs Arch Pharmacol; 5-aminosalicylic acid alleviates colitis and protects intestinal barrier function by modulating gut microbiota in mice; Authors - Jingjing Wang
mouse	IF	Front Nutr; Lactobacillus fermentum CECT5716 Alleviates the Inflammatory Response in Asthma by Regulating TLR2/TLR4 Expression; Authors - Weifang Wang
mouse	IF	Diabetes Metab Syndr Obes; Effects of Different Carbohydrate Content Diet on Gut Microbiota and Aortic Calcification in Diabetic Mice; Authors - Xinyi Shen