Claudin 5 Polyklonaler Antikörper

Claudin 5 Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(29)

Kat-Nr. : 29767-1-AP

Synonyme

CLDN5, AWAL, BEC1, Claudin5, Claudin-5

---

• 

  WB analysis using 29767-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29767-1-AP (Claudin 5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 29767-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29767-1-AP (Claudin 5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 29767-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29767-1-AP (Claudin 5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse lung using 29767-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse lung tissue using Claudin 5 antibody (29767-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: unboiled mouse lung tissue, unboiled rat lung tissue
• Erfolgreiche Detektion in IHC: humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Mauslungengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 27 publications below
• IF: See 4 publications below

Produktinformation

29767-1-AP bindet in WB, IHC, IF-P, ELISA Claudin 5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Claudin 5 fusion protein Ag29044
• Vollständiger Name: claudin 5
• Berechnetes Molekulargewicht: 218 aa, 23 kDa
• Beobachtetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: BC032363
• Gene symbol: CLDN5
• Gene ID (NCBI): 7122
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. 27 claudins have been identified. They are small (20-27 kilodalton (kDa)) proteins with very similar structure. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm.

Publikationen

Species	Application	Title
mouse	WB	Adv Healthc Mater; Colon-Targeted Ginseng Polysaccharides-Based Microspheres for Improving Ulcerative Colitis via Anti-Inflammation and Gut Microbiota Modulation; Authors - De-Yang Huo
mouse	WB	Microbiol Res; Trojan horse strategy and TfR/ LDLR-Mediated transcytosis determine the dissemination of mycobacteria in tuberculous meningoencephalitis; Authors - Ziyi Liu
mouse	WB	Life Sci; Metabolomics study reveals increased deoxycholic acid contributes to deoxynivalenol-mediated intestinal barrier injury; Authors - Xin He
rat	WB	Int J Mol Sci; Ursolic Acid Alleviates Neuroinflammation after Intracerebral Hemorrhage by Mediating Microglial Pyroptosis via the NF-κB/NLRP3/GSDMD Pathway; Authors - Pan Lei
rat,human	WB	J Ethnopharmacol; Houshiheisan modulates the NF-κB/MLCK signaling pathway to protect the endothelial barrier in cerebral small vessel disease; Authors - Hong-Fa Cheng
mouse	WB	Exp Neurol; Inhibition of histamine receptor 3 alleviates sevoflurane-induced hypomyelination and neurobehavioral deficits; Authors - Ji Che