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Claudin 5 Polyklonaler Antikörper

Claudin 5 Polyklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29767-1-PBS

Synonyme

CLDN5, AWAL, BEC1, Claudin5, Claudin-5

Filter:
  • Western Blot (WB) analysis of various lysates using Claudin 5 Polyclonal antibody (29767-1-AP)

    WB analysis using 29767-1-AP (same clone as 29767-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29767-1-AP (Claudin 5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29767-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Claudin 5 Polyclonal antibody (29767-1-AP)

    IHC staining of human colon using 29767-1-AP (same clone as 29767-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29767-1-AP (Claudin 5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29767-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Claudin 5 Polyclonal antibody (29767-1-AP)

    IHC staining of human colon using 29767-1-AP (same clone as 29767-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29767-1-AP (Claudin 5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29767-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse lung tissue using Claudin 5 Polyclonal antibody (29767-1-AP)

    IF Staining of mouse lung using 29767-1-AP (same clone as 29767-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse lung tissue using Claudin 5 antibody (29767-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29767-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

29767-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA Claudin 5 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Claudin 5 fusion protein Ag29044
Vollständiger Name claudin 5
Berechnetes Molekulargewicht 218 aa, 23 kDa
Beobachtetes Molekulargewicht22 kDa
GenBank-ZugangsnummerBC032363
Gene symbol CLDN5
Gene ID (NCBI) 7122
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. 27 claudins have been identified. They are small (20-27 kilodalton (kDa)) proteins with very similar structure. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm.