Claudin 9 Polyklonaler Antikörper

Claudin 9 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30520-1-PBS

Synonyme

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  WB analysis using 30520-1-AP (same clone as 30520-1-PBS)

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 30520-1-AP (Claudin 9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30520-1-PBS in a different storage buffer formulation.

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  IHC staining of human endometrial cancer using 30520-1-AP (same clone as 30520-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 30520-1-AP (Claudin 9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30520-1-PBS in a different storage buffer formulation.

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  IHC staining of human endometrial cancer using 30520-1-AP (same clone as 30520-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 30520-1-AP (Claudin 9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30520-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 30520-1-AP (same clone as 30520-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30520-1-AP (Claudin 9 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30520-1-PBS in a different storage buffer formulation.

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  IF Staining of T-47D using 30520-1-AP (same clone as 30520-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed T-47D cells using Claudin 9 antibody (30520-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 30520-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

30520-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA Claudin 9 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Claudin 9 fusion protein Ag29607
• Vollständiger Name: claudin 9
• Berechnetes Molekulargewicht: 217 aa, 23 kDa
• Beobachtetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC051870
• Gene symbol: Claudin 9
• Gene ID (NCBI): 9080
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium.They are small (20-27kDa) proteins with very similar structure. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm. This antibody is specifically against claudin 9.