Cleaved Caspase 3/P17/P19 Rekombinanter Antikörper

Cleaved Caspase 3/P17/P19 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

CloneNo.

230527B2

Kat-Nr. : 82707-13-RR

Synonyme

CASP3, Caspase3, 230527B2, CASP 3, CASP-3

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  WB analysis using 82707-13-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82707-13-RR (Cleaved Caspase 3/P17/P19 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: mit Staurosporin behandelte Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Produktinformation

82707-13-RR bindet in WB, IHC, IF, ELISA Cleaved Caspase 3/P17/P19 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: caspase 3, apoptosis-related cysteine peptidase
• Berechnetes Molekulargewicht: 32 kDa
• Beobachtetes Molekulargewicht: 17 kDa, 19 kDa
• GenBank-Zugangsnummer: NM_032991
• Gene symbol: Caspase 3
• Gene ID (NCBI): 836
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can form heterocomplex with other proteins. This antibody can recognize p17 and p19 fragment of Caspase 3. This antibody is specific for cleaved caspase 3, and does not recognize full length caspase-3. The cleaved P17 and P19 fragment might form complex and shows at around 30-35 kDa by western blot (PMID: 25501826).

Publikationen

Species	Application	Title
mouse	WB,IF	Arch Biochem Biophys; DANCR Knockdown Alleviates Neuroinflammation and Functional Recovery after Spinal Cord Injury via Regulating the ACTN4 / STAT3 Axis; Authors - Bin Xia
mouse	WB	CNS Neurosci Ther; CHOP-Mediated Disruption of Hippocampal Synaptic Plasticity and Neuronal Activity Contributes to Chronic Pain-Related Cognitive Deficits; Authors - Qingsheng Meng
mouse	WB	J Membr Biol; Schisandrin B Improves Mitochondrial Function and Inhibits HT22 Cell Apoptosis by Regulating Sirt3 Protein; Authors - Fei Hu
human	WB	Toxicol Appl Pharmacol; Exploring the mechanism of baicalein on breast cancer based on network pharmacology, molecular docking and in vivo experiments; Authors - Gaotao Zhang
human	WB	Bioorg Chem; Design, synthesis and antitumor activity evaluation of novel modified 18β-glycyrrhetinate derivatives as PPARγ agonists; Authors - Hongyan Lin
human	WB	J Med Chem; Discovery of Novel Oxazolo[4,3-f]purine Derivatives as Antitumor Agents through PPIA Interaction; Authors - Xian-Jia Li