Cleaved PARP1 (Asp214) Rekombinanter Antikörper

Cleaved PARP1 (Asp214) Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

243142B10

Kat-Nr. : 85175-5-PBS

Synonyme

PARP1, ADP-ribosyltransferase diphtheria toxin-like 1, ADPRT, ADPRT 1, ADPRT1

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  WB analysis of HeLa using 85175-5-RR (same clone as 85175-5-PBS)

  Staurosporine treated HeLa cells were subjected to SDS PAGE followed by western blot with 85175-5-RR (Cleaved PARP1 (Asp214) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85175-5-PBS in a different storage buffer formulation.

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  WB analysis using 85175-5-RR (same clone as 85175-5-PBS)

  Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 85175-5-RR (Cleaved PARP1 (Asp214) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85175-5-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

85175-5-PBS bindet in WB, Indirect ELISA Cleaved PARP1 (Asp214) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: poly (ADP-ribose) polymerase 1
• Berechnetes Molekulargewicht: 1014 aa, 113 kDa
• Beobachtetes Molekulargewicht: 89 kDa
• GenBank-Zugangsnummer: BC037545
• Gene symbol: PARP1
• Gene ID (NCBI): 142
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PARP1 (poly(ADP-ribose) polymerase 1) is a nuclear enzyme catalyzing the poly(ADP-ribosyl)ation of many key proteins in vivo. The normal function of PARP1 is the routine repair of DNA damage. Activated by DNA strand breaks, the PARP1 is cleaved into an 85 to 89-kDa COOH-terminal fragment and a 24 kDa NH2-terminal peptide by caspases during the apoptotic process. The appearance of PARP fragments is commonly considered an important biomarker of apoptosis. In addition to caspases, other proteases like calpains, cathepsins, granzymes, and matrix metalloproteinases (MMPs) have also been reported to cleave PARP1 and give rise to fragments ranging from 42-89 kDa.
This antibody only recognizes the cleaved form of PAPR1 but not full-length PARP1.