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Clusterin Rekombinanter Antikörper

Clusterin Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241277D4

Kat-Nr. : 84067-4-PBS

Synonyme

Apo-J, APOJ, Apo J, Aging-associated gene 4 protein, AAG4

Filter:
  • Western Blot (WB) analysis of human plasma using Clusterin Recombinant antibody (84067-4-RR)

    WB analysis of human plasma using 84067-4-RR (same clone as 84067-4-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 84067-4-RR (Clusterin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84067-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Clusterin Recombinant antibody (84067-4-RR)

    IF Staining of HeLa using 84067-4-RR (same clone as 84067-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Clusterin antibody (84067-4-RR, Clone: 241277D4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84067-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84067-4-RR

    Affinity and Kinetic Characterization of 84067-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84067-4-RR against Human Clusterin were performed. The affinity constant is 6.91 nM.

Geprüfte Anwendungen

Produktinformation

84067-4-PBS bindet in WB, IF/ICC, Indirect ELISA Clusterin und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Fusionsprotein
Vollständiger Name clusterin
Berechnetes Molekulargewicht 449 aa, 53 kDa
Beobachtetes Molekulargewicht37 kDa
GenBank-ZugangsnummerBC010514
Gene symbol CLU
Gene ID (NCBI) 1191
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Clusterin (Apolipoprotein J) is a 75-80 kDa heterodimeric glycoprotein produced by a wide array of tissues and found in most biologic fluids (PMID: 7648419; 11906815). It is encoded by one single gene and cleaved posttranslationally into α (34-36 kDa) and β (36-39 kDa) subunits prior to secretion from the cell. Many functions have been described for clusterin, including regulating apoptosis, transporting lipids, controlling cell interactions, regulating complement (PMID: 10694874).