Coagulation Factor II Polyklonaler Antikörper

Coagulation Factor II Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 31615-1-AP

Synonyme

F2, Prothrombin, Activation peptide fragment 1, Activation peptide fragment 2, PT

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  WB analysis using 31615-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31615-1-AP (Coagulation Factor II antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis using 31615-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31615-1-AP (Coagulation Factor II antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 31615-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 31615-1-AP (Coagulation Factor II antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 31615-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 31615-1-AP (Coagulation Factor II antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 31615-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Coagulation Factor II antibody (31615-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: human plasma, Mausherzgewebe, Mauslebergewebe
• Erfolgreiche Detektion in IHC: Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

31615-1-AP bindet in WB, IHC, IF/ICC, ELISA Coagulation Factor II und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: coagulation factor II
• Berechnetes Molekulargewicht: 70 kDa
• Beobachtetes Molekulargewicht: 70-80 kDa
• GenBank-Zugangsnummer: NM_010168.3
• Gene symbol: F2
• Gene ID (NCBI): 14061
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Normal blood coagulation is a complex process, involving a cascade of activation of different plasma proteins, ultimately resulting in the formation of a clot, called fibrin. Coagulation Factor II (F2), also known as prothrombin or factor II, is one of the components of this chain of plasma proteins involved in blood coagulation. Prothrombin is the precursor of thrombin, which is essential in the processes of hemostasis and thrombosis. This antibody specifically recognizes mouse Coagulation Factor II.