Coagulation factor II Rekombinanter Antikörper

Coagulation factor II Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

243089F11

Kat-Nr. : 86124-1-RR

Synonyme

F2, Prothrombin, Activation peptide fragment 1, Activation peptide fragment 2, EC:3.4.21.5

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  WB analysis of human plasma using 86124-1-RR

  human plasma were subjected to SDS PAGE followed by western blot with 86124-1-RR (Prothrombin/F2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 86124-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86124-1-RR against Human Coagulation factor II were performed. The affinity constant is 54.6 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: human plasma

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86124-1-RR bindet in WB, ELISA Coagulation factor II und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Coagulation factor II fusion protein Eg1337
• Vollständiger Name: coagulation factor II (prothrombin)
• Berechnetes Molekulargewicht: 70kDa
• Beobachtetes Molekulargewicht: 72 kDa
• GenBank-Zugangsnummer: NM_000506.5
• Gene symbol: F2
• Gene ID (NCBI): 2147
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Normal blood coagulation is a complex process, involving a cascade of activation of different plasma proteins, ultimately resulting in the formation of a clot, called fibrin. Coagulation Factor II (F2), also known as prothrombin, is one of the components of this chain of plasma proteins involved in blood coagulation. Prothrombin is the precursor of thrombin, which is essential in the processes of hemostasis and thrombosis.