Collagen Type I Rekombinanter Antikörper

Collagen Type I Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

250534B1

Kat-Nr. : 86093-1-RR

Synonyme

COL1A1, Alpha 1 type I collagen, Alpha-1 type I collagen, COL I, col1

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  WB analysis of SK-N-SH using 86093-1-RR

  SK-N-SH cells were subjected to SDS PAGE followed by western blot with 86093-1-RR (COL1A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 86093-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 86093-1-RR (COL1A1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 86093-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 86093-1-RR (COL1A1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 86093-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86093-1-RR against Human Collagen Type I were performed. The affinity constant is 4.37 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SK-N-SH cells
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86093-1-RR bindet in WB, IHC, ELISA Collagen Type I und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Collagen Type I fusion protein Ag27389
• Vollständiger Name: collagen, type I, alpha 1
• Berechnetes Molekulargewicht: 1464 aa, 139 kDa
• Beobachtetes Molekulargewicht: 140 kDa, 200kDa
• GenBank-Zugangsnummer: BC036531
• Gene symbol: COL1A1
• Gene ID (NCBI): 1277
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Type I collagen, the major structural component of connective tissues such as skin, tendon and bone, is the most abundant and widely expressed collagen in humans (PMID: 7620364; 8645190; 9016532). Type I collagen is a heterotrimer comprising one alpha 2(I) and two alpha 1(I) chains which are encoded by the unlinked loci COL1A2 and COL1A1 respectively. Mutations in COL1A1 are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. This antibody raised against a synthesized peptide corresponding to 1206-1218 aa of human pro-alpha 1 chain of type I collagen recognize collagen alpha-1(I) chain. The presence of unprocessed, intermediate, and mature chains of type I collagen was clearly detected only in static constructs. Indeed, in sponges cultured under perfusion the presence of type I collagen was mainly restricted to mature chains, suggesting that HACs were no longer actively producing type I collagen (PMID: 27584727). It is reported that the molecular weight is 200kDa(PMID: 28526934).