Collagen Type I Monoklonaler Antikörper

Collagen Type I Monoklonal Antikörper für IF-P

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

IF-P

Konjugation

CoraLite®594 Fluorescent Dye

Publikationen(1)

CloneNo.

1E9A7

Rezensionen (1)

Kat-Nr. : CL594-67288

Synonyme

COL1A1, col1, COL I, Alpha-1 type I collagen, Alpha 1 type I collagen

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  IF Staining of human colon cancer using CL594-67288

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®594 Collagen Type I antibody (CL594-67288, Clone: 1E9A7 ) at dilution of 1:200.

• 

  IF Staining of human colon cancer using CL594-67288

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®594 Collagen Type I antibody (CL594-67288, Clone: 1E9A7 ) at dilution of 1:200.

• 

  IF Staining of mouse skin using CL594-67288

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using CoraLite®594 Collagen Type I antibody (CL594-67288, Clone: 1E9A7 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human breast cancer using CL594-67288

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®594 Collagen Type I antibody (CL594-67288, Clone: 1E9A7 ) at dilution of 1:200.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: humanes Kolonkarzinomgewebe, humanes Mammakarzinomgewebe, Maushautgewebe

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

CL594-67288 bindet in IF-P Collagen Type I und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: collagen, type I, alpha 1
• Berechnetes Molekulargewicht: 139 kDa
• Beobachtetes Molekulargewicht: 120-130 kDa
• GenBank-Zugangsnummer: NM_000088
• Gene symbol: COL1A1
• Gene ID (NCBI): 1277
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Type I collagen, the major structural component of connective tissues such as skin, tendon and bone, is the most abundant and widely expressed collagen in humans (PMID: 7620364; 8645190; 9016532). Type I collagen is a heterotrimer comprising one alpha 2(I) and two alpha 1(I) chains which are encoded by the unlinked loci COL1A2 and COL1A1 respectively. Mutations in COL1A1 are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. This antibody raised against a synthesized peptide corresponding to 1206-1218 aa of human pro-alpha 1 chain of type I collagen recognize collagen alpha-1(I) chain.

Publikationen

Species	Application	Title
human	IF	Int J Biol Sci; Bulk and single-cell transcriptome profiling reveal extracellular matrix mechanical regulation of lipid metabolism reprograming through YAP/TEAD4/ACADL axis in hepatocellular carcinoma; Authors - Jingwei Cai

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Areli (Verified Customer) (03-14-2023)

I would use a higher dilution next time as the signal was high.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Y201-MSC cells

CL594 Collagen Type I Antibody Immunofluorescence validation (1:500 dilution) in Y201-MSC cells (Cat no:CL594-67288)