Collagen Type II Polyklonaler Antikörper

Collagen Type II Polyklonal Antikörper für IHC, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (4)

Anwendung

WB, IHC, IF/ICC, IF-P, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(363)

Rezensionen (2)

Kat-Nr. : 28459-1-AP

Synonyme

COL2, COL2A1, Alpha 1 type II collagen, Alpha-1 type II collagen, ANFH

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  IHC staining of human lung using 28459-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 28459-1-AP (Collagen Type II antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 28459-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 28459-1-AP (Collagen Type II antibody) at dilution of 1:1600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human lung cancer using 28459-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using Collagen Type II antibody (28459-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of human lung cancer using 28459-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using Collagen Type II antibody (28459-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of ATDC-5 using 28459-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed ATDC-5 cells using Collagen Type II antibody (28459-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Lungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Lungenkarzinomgewebe
• Erfolgreiche Detektion in IF/ICC: ATDC-5-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:800-1:3200
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 218 publications below
• IHC: See 142 publications below
• IF: See 150 publications below
• CoIP: See 2 publications below

Produktinformation

28459-1-AP bindet in WB, IHC, IF/ICC, IF-P, CoIP, ELISA Collagen Type II und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Hausschwein, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Collagen Type II fusion protein Ag29265
• Vollständiger Name: collagen, type II, alpha 1
• Berechnetes Molekulargewicht: 134 kDa
• GenBank-Zugangsnummer: BC007252
• Gene symbol: COL2A1
• Gene ID (NCBI): 1280
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The COL2A1 gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found primarily in cartilage and the vitreous humor of the eye. Type II collagen is essential for the formation of the extracellular matrix in cartilage and other connective tissues, providing structure and strength to these tissues .

Publikationen

Species	Application	Title
mouse,human	IHC,IF	Nature; A plant-derived natural photosynthetic system for improving cell anabolism; Authors - Pengfei Chen
rat	IF	Adv Mater; Injectable Microgels with Hybrid Exosomes of Chondrocyte-Targeted FGF18 Gene-Editing and Self-Renewable Lubrication for Osteoarthritis Therapy; Authors - Manyu Chen
rat	IHC	Bioact Mater; Engineering a stem cell-embedded bilayer hydrogel with biomimetic collagen mineralization for tendon-bone interface healing; Authors - Tingyun Lei
mouse,rat	IHC,IF	Bioact Mater; Synergizing adaptive immunity and regenerative signals to enhance osteochondral defects repair; Authors - Changjian Lin
rat	IF	Nat Commun; Injectable ECM-mimetic dynamic hydrogels abolish ferroptosis-induced post-discectomy herniation through delivering nucleus pulposus progenitor cell-derived exosomes; Authors - Wenkai Wang
		Adv Sci (Weinh); Silencing of NOTCH3 Signaling in Meniscus Smooth Muscle Cells Inhibits Fibrosis and Exacerbates Degeneration in a HEYL-Dependent Manner; Authors - Hao Sun

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Steve (Verified Customer) (07-23-2025)

I used that antibody in flow to detect cells from canine species, so far so good. I am validating at this moment in Western Blot.

• Applications: Western Blot, Flow Cytometry
• Cell Tissue Type: Stem Cell

FH

Balawant (Verified Customer) (12-20-2022)

Ive used this Antibody. this great antibody for IHC.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: colon Tissue