Collagen Type II Polyklonaler Antikörper

Collagen Type II Polyklonal Antikörper für IF, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (4)

Anwendung

WB, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

184

Rezensionen (1)

Kat-Nr. : 28459-1-AP

Synonyme

Alpha 1 type II collagen, ANFH, AOM, COL11A3, COL2A1, Col2α1, Collagen alpha 1(II) chain, Collagen Type II, collagen, type II, alpha 1, SEDC

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Galerie der Validierungsdaten

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  IHC staining of human lung using 28459-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 28459-1-AP (Collagen Type II antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 28459-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 28459-1-AP (Collagen Type II antibody) at dilution of 1:1600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human lung cancer using 28459-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using Collagen Type II antibody (28459-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of human lung cancer using 28459-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using Collagen Type II antibody (28459-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Lungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Lungenkarzinomgewebe

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:800-1:3200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 122 publications below
• IHC: See 75 publications below
• IF: See 62 publications below
• CoIP: See 2 publications below

Produktinformation

28459-1-AP bindet in WB, IHC, IF, CoIP, ELISA Collagen Type II und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Hausschwein, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Collagen Type II fusion protein Ag29265
• Vollständiger Name: collagen, type II, alpha 1
• Berechnetes Molekulargewicht: 134 kDa
• GenBank-Zugangsnummer: BC007252
• Gene symbol: COL2A1
• Gene ID (NCBI): 1280
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human,mouse	IHC,IF	Nature; A plant-derived natural photosynthetic system for improving cell anabolism; Authors - Pengfei Chen
human	WB	Nat Commun; Dual functions of microRNA-17 in maintaining cartilage homeostasis and protection against osteoarthritis.; Authors - Yun Zhang
human	WB	J Musculoskelet Neuronal Interact; Experimental study of miR-503 regulating the activity as well as the function of degenerated human nucleus pulposus cells of the intervertebral disc through inhibiting Wnt pathway; Authors - Xiaoming Shi
rabbit	IHC	Acta Biomater; The characterization, cytotoxicity, macrophage response and tissue regeneration of decellularized cartilage.; Authors - Wang Changchen
mouse	WB	J Nanobiotechnology; Injectable photocrosslinking spherical hydrogel-encapsulated targeting peptide-modified engineered exosomes for osteoarthritis therapy; Authors - Junlai Wan
human	WB	Mol Ther; Circular RNA circPDE4D Protects against Osteoarthritis by Binding to miR-103a-3p and Regulating FGF18.; Authors - Yizheng Wu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Balawant (Verified Customer) (12-20-2022)

Ive used this Antibody. this great antibody for IHC.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: colon Tissue