Collagen Type III (N-terminal) Rekombinanter Antikörper

Collagen Type III (N-terminal) Rekombinant Antikörper für IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF

Konjugation

CoraLite®555 Fluorescent Dye

CloneNo.

2O1

Kat-Nr. : CL555-80009

Synonyme

COL3A1, Collagen alpha 1(III) chain, collagen, type III, alpha 1, EDS4A, FLJ34534

---

Galerie der Validierungsdaten

• 

  IF Staining of human colon using CL555-80009

  Immunofluorescent analysis of (4% PFA) fixed human colon tissue using CoraLite®555 Collagen Type III (N-terminal) antibody (CL555-80009, Clone: 2O1 ) at dilution of 1:200.

• 

  IF Staining of human colon using CL555-80009

  Immunofluorescent analysis of (4% PFA) fixed human colon tissue using CoraLite®555 Collagen Type III (N-terminal) antibody (CL555-80009, Clone: 2O1 ) at dilution of 1:200.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: humanes Kolongewebe

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL555-80009 bindet in IF Collagen Type III (N-terminal) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Collagen Type III (N-terminal) fusion protein Ag18658
• Vollständiger Name: collagen, type III, alpha 1
• Berechnetes Molekulargewicht: 1466 aa, 139 kDa
• Beobachtetes Molekulargewicht: 140-180 kDa
• GenBank-Zugangsnummer: BC028178
• Gene symbol: COL3A1
• Gene ID (NCBI): 1281
• Konjugation: CoraLite®555 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 557 nm / 570nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Type III collagen is a fibrillar forming collagen comprising three α1(III) chains and is expressed in early embryos and throughout embryogenesis (PMID: 9050868). In the adult, type III collagen is a major component of the extracellular matrix in a variety of internal organs and skin. It occurs in most soft connective tissues along with type I collagen (PMID: 2445760). COL3A1 gene encodes type III procollagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms (PMID: 10706896; 2243125; 18389341). This antibody was raised against 24-152 aa of prepro α1 (III) chain of human type III procollagen. It can't recognize mouse or rat type III collagen.