COL4A1 Polyklonaler Antikörper

COL4A1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 30850-1-AP

Synonyme

arresten, Collagen alpha 1(IV) chain, Collagen alpha-1(IV) chain, Collagen Type IV

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  WB analysis using 30850-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30850-1-AP (COL4A1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of Hepatocellular carcinoma using 30850-1-AP

  Immunohistochemical analysis of paraffin-embedded Hepatocellular carcinoma tissue slide using 30850-1-AP (COL4A1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, SK-N-SH cells
• Erfolgreiche Detektion in IHC: Hepatocellular carcinoma tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below

Produktinformation

30850-1-AP bindet in WB, IHC, ELISA COL4A1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: COL4A1 fusion protein Ag34391
• Vollständiger Name: collagen, type IV, alpha 1
• Berechnetes Molekulargewicht: 1669 aa, 161 kDa
• Beobachtetes Molekulargewicht: 160-220 kDa
• GenBank-Zugangsnummer: BC151220
• Gene symbol: COL4A1
• Gene ID (NCBI): 1282
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

COL4A1 (Collagen Type IV Alpha 1 Chain) encodes a major component of type IV collagen, which is an essential structural element of basement membranes in various tissues. COL4A1 forms heterotrimers with other collagen type IV chains (e.g., COL4A2) and interacts with extracellular matrix components such as laminins, proteoglycans, and perlecans. Proteolytic cleavage of the non-collagenous domain of COL4A1 produces a fragment called arresten, which has anti-angiogenic and tumor suppressor properties. Elevated expression of COL4A1 has been observed in various cancers, including hepatocellular carcinoma (HCC), where it promotes tumor growth and metastasis through the FAK-Src signaling pathway. COL4A1 is highly expressed in various tissues, including the placenta, fat, and other organs. The expression of COL4A1 can be regulated by transcription factors such as RUNX1, which has been shown to upregulate COL4A1 in hepatocellular carcinoma. The molecular weight of OSBPL5 is 160 kDa, and it also has glycosylation modification.

Publikationen

Species	Application	Title
mouse	IHC	Sci Rep; Yiqi Juanshen decoction alleviates renal interstitial fibrosis by targeting the LOXL2/PI3K/AKT pathway to suppress EMT and inflammation; Authors - Kaiyue Tan