COL4A2 Polyklonaler Antikörper

COL4A2 Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(71)

Kat-Nr. : 55131-1-AP

Synonyme

COL4A2-specific, Canstatin, Collagen Ⅳ, Collagen alpha-2(IV) chain, Collagen Type IV



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHeLa-Zellen
Erfolgreiche Detektion in IHChumanes Nierengewebe, humanes Mammakarzinomgewebe, humanes Mammahyperplasie-Gewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF-Phumanes Nierengewebe

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:6000
Immunhistochemie (IHC)IHC : 1:1000-1:4000
Immunfluoreszenz (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

55131-1-AP bindet in WB, IHC, IF-P, ELISA COL4A2 und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman, Hausschwein
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name collagen, type IV, alpha 2
Berechnetes Molekulargewicht 168 kDa
Beobachtetes Molekulargewicht200 kDa
GenBank-ZugangsnummerNM_001846
Gene symbol COL4A2
Gene ID (NCBI) 1284
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for COL4A2 antibody 55131-1-APProtokoll herunterladen
IHC protocol for COL4A2 antibody 55131-1-APProtokoll herunterladenl
IF protocol for COL4A2 antibody 55131-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle

EMBO J

Proline biosynthesis is a vent for TGFβ-induced mitochondrial redox stress.

Authors - Simon Schwörer
human

EBioMedicine

Aspirin targets P4HA2 through inhibiting NF-κB and LMCD1-AS1/let-7g to inhibit tumour growth and collagen deposition in hepatocellular carcinoma.

Authors - Tianjiao Wang
IF

ACS Appl Mater Interfaces

Novel Thermosensitive Hydrogel Promotes Spinal Cord Repair by Regulating Mitochondrial Function.

Authors - Yi Li
humanIF

Biomaterials

Tumor-specific nitric oxide generator to amplify peroxynitrite based on highly penetrable nanoparticles for metastasis inhibition and enhanced cancer therapy.

Authors - Menghao Shi
humanIHC

Biofabrication

Engineering microvasculature by 3D bioprinting of prevascularized spheroids in photo-crosslinkable gelatin.

Authors - Lise De Moor

Cell Mol Life Sci

Macrophage Dectin-1 mediates Ang II renal injury through neutrophil migration and TGF-β1 secretion

Authors - Shiju Ye

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Samantha (Verified Customer) (05-10-2024)

Clean bands detected in DRG and Spinal cord tissue of mice.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1 in 1000 and 1 in 5000
  • Cell Tissue Type: Dorsal Root Ganglion, Spinal Cord
FH

Pankhuri (Verified Customer) (08-22-2022)

Nice clean antibody. Did IF for collagen in NSCs organoid. The collagen fibres in the ECM of organoid were stained beautifully.

  • Applications: Immunofluorescence
  • Cell Tissue Type: NSC organoid
COL4A2 Antibody Immunofluorescence validation ( dilution) in NSC organoid (Cat no:55131-1-AP)
FH

Lindsey (Verified Customer) (05-24-2021)

BULLETPROOF ANTIBODY!

  • Applications: Immunohistochemistry, Immunofluorescence
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Human kidney (FFPE)
COL4A2 Antibody Immunohistochemistry,Immunofluorescence validation (1:500 dilution) in Human kidney (FFPE) (Cat no:55131-1-AP)
FH

Malak (Verified Customer) (02-24-2021)

This antibody works very well

  • Applications: Western Blot
FH

Joshua (Verified Customer) (04-28-2019)

Vascular endothelial cells in 3D culture were fixed in paraformaldehdye and stained for collagen IV (red). DRAQ7 used as a counterstain (blue). As expected, collagen IV localized to the vascular basement membranes.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: HMEC1
COL4A2 Antibody Immunofluorescence, validation (1:500 dilution) in HMEC1 (Cat no:55131-1-AP)