COL4A2 Polyklonaler Antikörper
COL4A2 Polyklonal Antikörper für WB, IHC, IF-P, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human und mehr (1)
Anwendung
WB, IHC, IF-P, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 55131-1-AP
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | HeLa-Zellen | 
| Erfolgreiche Detektion in IHC | humanes Nierengewebe,  humanes Mammakarzinomgewebe,  humanes Mammahyperplasie-Gewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. | 
| Erfolgreiche Detektion in IF-P | humanes Nierengewebe | 
Empfohlene Verdünnung
| Anwendung | Verdünnung | 
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 | 
| Immunhistochemie (IHC) | IHC : 1:1000-1:4000 | 
| Immunfluoreszenz (IF)-P | IF-P : 1:50-1:500 | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| IHC | See 22 publications below | 
| IF | See 14 publications below | 
Produktinformation
55131-1-AP bindet in WB, IHC, IF-P, ELISA COL4A2 und zeigt Reaktivität mit human
| Getestete Reaktivität | human | 
| In Publikationen genannte Reaktivität | human, Hausschwein | 
| Wirt / Isotyp | Kaninchen / IgG | 
| Klonalität | Polyklonal | 
| Typ | Antikörper | 
| Immunogen | Peptid | 
| Vollständiger Name | collagen, type IV, alpha 2 | 
| Berechnetes Molekulargewicht | 168 kDa | 
| Beobachtetes Molekulargewicht | 200 kDa | 
| GenBank-Zugangsnummer | NM_001846 | 
| Gene symbol | COL4A2 | 
| Gene ID (NCBI) | 1284 | 
| Konjugation | Unkonjugiert | 
| Form | Liquid | 
| Reinigungsmethode | Antigen-Affinitätsreinigung | 
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol | 
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. | 
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for COL4A2 antibody 55131-1-AP | Protokoll herunterladen | 
| IHC protocol for COL4A2 antibody 55131-1-AP | Protokoll herunterladenl | 
| IF protocol for COL4A2 antibody 55131-1-AP | Protokoll herunterladen | 
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen | 
Publikationen
| Species | Application | Title | 
|---|---|---|
| EBioMedicine Aspirin targets P4HA2 through inhibiting NF-κB and LMCD1-AS1/let-7g to inhibit tumour growth and collagen deposition in hepatocellular carcinoma. | ||
| ACS Appl Mater Interfaces Novel Thermosensitive Hydrogel Promotes Spinal Cord Repair by Regulating Mitochondrial Function. | ||
| Biomaterials Tumor-specific nitric oxide generator to amplify peroxynitrite based on highly penetrable nanoparticles for metastasis inhibition and enhanced cancer therapy. | ||
| Biofabrication Engineering microvasculature by 3D bioprinting of prevascularized spheroids in photo-crosslinkable gelatin. | ||
| Cell Mol Life Sci Macrophage Dectin-1 mediates Ang II renal injury through neutrophil migration and TGF-β1 secretion | 
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
| FH Samantha (Verified Customer) (05-10-2024) | Clean bands detected in DRG and Spinal cord tissue of mice. 
 | 
| FH Pankhuri (Verified Customer) (08-22-2022) | Nice clean antibody. Did IF for collagen in NSCs organoid. The collagen fibres in the ECM of organoid were stained beautifully. 
  | 
| FH Lindsey (Verified Customer) (05-24-2021) | BULLETPROOF ANTIBODY! 
  | 
| FH Malak (Verified Customer) (02-24-2021) | This antibody works very well 
 | 
| FH Joshua (Verified Customer) (04-28-2019) | Vascular endothelial cells in 3D culture were fixed in paraformaldehdye and stained for collagen IV (red). DRAQ7 used as a counterstain (blue). As expected, collagen IV localized to the vascular basement membranes. 
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