COL5A1 Monoklonaler Antikörper

COL5A1 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(11)

CloneNo.

2C2C2

Kat-Nr. : 67604-1-Ig

Synonyme

2C2C2, Collagen alpha 1(V) chain, Collagen alpha-1(V) chain, Collagen Type V

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  WB analysis of HT-1080 using 67604-1-Ig

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 67604-1-Ig (Collagen Type V antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 67604-1-Ig

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 67604-1-Ig (Collagen Type V antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67604-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67604-1-Ig (Collagen Type V antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67604-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67604-1-Ig (Collagen Type V antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 67604-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67604-1-Ig (Collagen Type V antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67604-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67604-1-Ig (Collagen Type V antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 67604-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using Collagen Type V antibody (67604-1-Ig, Clone: 2C2C2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-1080.Zellen, HeLa-Zellen, Maus-Kolongewebe, Hausschwein-Kolongewebe, Ratten-Kolongewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 5 publications below
• IF: See 5 publications below

Produktinformation

67604-1-Ig bindet in WB, IHC, IF/ICC, ELISA COL5A1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: native protein
• Vollständiger Name: collagen, type V, alpha 1
• Berechnetes Molekulargewicht: 184 kDa
• Beobachtetes Molekulargewicht: 220 kDa
• GenBank-Zugangsnummer: NM_000093
• Gene symbol: COL5A1
• Gene ID (NCBI): 1289
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IF	Theranostics; Dysregulation of interaction between LOXhigh fibroblast and smooth muscle cells contributes to the pathogenesis of aortic dissection.; Authors - Yinan Chen
human	IHC	Exp Ther Med; COL3A1, COL5A1 and COL6A2 serve as potential molecular biomarkers for osteoarthritis based on weighted gene co‑expression network analysis bioinformatics analysis; Authors - Yufeng Zhang
human	IHC	Neuropathology; Immunohistochemical expression of osteopontin and collagens in choroid plexus of human brains.; Authors - Keiji Wakamatsu
human	IF	Nat Cardiovasc Res; Single-cell spatial transcriptomics of tertiary lymphoid organ-like structures in human atherosclerotic plaques; Authors - Zhichao Lai
human	WB,IHC,IF	Comput Biol Med; Identification and targeting of cancer-associated fibroblast signature genes for prognosis and therapy in Cutaneous melanoma; Authors - Guokun Zhang
		J Adv Res; Mesenteric adipose-derived exosomal TINAGL1 enhances intestinal fibrosis in Crohn's Disease via SMAD4; Authors - Yidong Chen